不同诱导方法对人外周血树突状细胞体外成熟的影响

目的探讨不同诱导方式对于体外培养的树突状细胞（dendritic cells，DC）免疫刺激活性的影响。方法通过rhGM-CSF和IL-4体外诱导的人外周血单核细胞来源的DC，分别用肿瘤坏死因子α（TNF-α）、脂多糖（LPS）、细胞因子鸡尾酒法诱导成熟，再以流式细胞仪、FITC－Dxtran内吞检测、MLR、ELISA法检测DC的表面标志、内吞能力、刺激淋巴细胞增殖能力和IL－12分泌的变化。结果在不同方法促进DC成熟中，细胞因子鸡尾酒法诱导下的DC成熟状态最佳，CD83指数表达高达84．87％，IL-12的分泌量[（656．18±38．52ng／L）]高于其他各组，且刺激淋巴细胞增殖能力最强（P〈0．05）。结论细胞因子鸡尾酒法是体外诱导DC成熟的最佳方法。

Different cytokines induce the maturation of human monocyte-derived dendritic cells

Objective To investigate the immunity effect of different cytokine on immature dendritic cells in vitro.Methods The human monocyte-derived dendritic cells were induced in the presence of cytokine recombined human GM-CSF and interleukin(IL)-4.The effects of TNF-α,LPS,the cocktail of cytokines(TNF-α,IL-6,IL-1β,PGE2) on the dendritic cells were detected by the FCM,endocytic activity,T cells stimulatory proliferation capacity,and IL-12p40 production.Results Among the stimulus,the cocktail of cytokines could up-regulate the mature state of dendritic cells,and CD83 expression was 84.87%.Furthermore,the T helper cell type 1-skewing factor IL-12 production was 656.18 ± 38.52,and the ability of T-cell stimulation was up-regulated in the dendritic cells of cocktail group.Conclusion The most effective method for inducing human monocyte-derived dendritic cells maturation is cocktail of cytokines.