| 地塞米松对大鼠睾丸间质细胞睾酮分泌的影响及机理研究 |
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| 引用本文： | 肖爱娇,王昌磊,方廉.地塞米松对大鼠睾丸间质细胞睾酮分泌的影响及机理研究[J].江西医学院学报,2004,44(1):32-35. |
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| 作者姓名： | 肖爱娇 王昌磊 方廉 |
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| 作者单位： | 江西医学院生理学教研室，江西南昌330006 |
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| 基金项目： | 江西省自然科学基金项目 (0 0 4 0 0 6 9)资助 |
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| 摘 要： | 目的 观察地塞米松(dexamethasone，DEX)以及DEX和RU486共同作用时对hCG诱导的大鼠间质细胞睾酮分泌的影响和间质细胞中原癌基因c-myb表达的情况，从而探讨DEX的作用机制。方法 用放射免疫方法测定离体大鼠间质细胞的睾酮分泌量；用S-P免疫组织化学方法观察离体大鼠间质细胞中c-Myb蛋白表达的变化。结果 10^-8mol／L的DEX可降低hCG诱导的离体大鼠间质细胞睾酮分泌．并使间质细胞c-Myb蛋白免疫组织化学染色明显下降。上述DEX作用可被糖皮质激素受体功能阻断剂RU486(10μmol／L)所阻断。结论 DEX很可能通过降低间质细胞中c-Myb蛋白表达而抑制hCG诱导的间质细胞睾酮分泌。
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| 关 键 词： | 间质细胞 地塞米松 c-myb 睾酮 |
| 文章编号： | 1000-2294(2004)01-0032-04 |
| 修稿时间： | 2003年9月27日 |
Experimental Study of Effect of Dexamethasone on Testosterone Secretion in Isolated Rat Leydig Cells |
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| XIAO Ai-jiao,WANG Jing-lei,FANG Lian.Experimental Study of Effect of Dexamethasone on Testosterone Secretion in Isolated Rat Leydig Cells[J].Acta Academiae Medicinae Jiangxi,2004,44(1):32-35. |
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| Authors: | XIAO Ai-jiao WANG Jing-lei FANG Lian |
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| Abstract: | Objective To investigate the effects of dexamethasone(DEX) alone and in combination with RU486 on hCG-induced testosterone secretion and c-myb expression in isolated rat Leydig cells.The mechanism of dexamethasone was also analyzed.Methods (1)Testosterone secretion of isolated rat Leydig cells was measured by radioimmunoassay.(2)C-Myb protein of isolated rat Leydig cells was detected by immunohistochemistry.Results DEX(10 -8mol/L)inhibited hCG-induced testosterone secretion in isolated rat Leydig cells.And integral optical density(IOD)immustaining of Myb in Leydig cells was also remarkably reduced.These effects could be blocked by RU486(10 -8mol/L),a dexamethasone antagonist.Conclusion The results indicated that DEX can decrease hCG-induced testosterone secretion,which might be through inhibiting c-Myb synthesis of Leydig cells. |
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| Keywords: | leydig cells dexamethasone c-myb testosterone |
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