FHIT在散发性结肠癌中的表达及意义

目的探讨散发性结肠癌中脆性组氨酸三联体(FHIT)基因蛋白表达状况及其与临床病理指标的可能关系。方法采用兔抗人FHIT蛋白抗体和枸橼酸微波SP免疫组织化学方法检测74例结肠癌26例正常组织、26例良性腺瘤标本中FHIT表达状况并分析其与Dukes’分期等临床病理指标以及5年生存率的关系。结果26例正常结肠组织中FHIT基因表达,1例阴性(1/26,3.8%)26例结肠腺瘤组织阴性7例(7/26,26.9%)。74例结肠癌组织中阴性35例(47.3%)。三者比较有显著性差异(P<0.05)。FHIT蛋白低表达与结肠癌患者的年龄、性别、肿瘤部位、大小、血清CEA的水平、组织学类型均无相关性(P>0.05);与分化程度、病理分期、淋巴转移、远处转移、五年生存率均有差异(P<0.05)。结论FHIT表达降低可能对结肠癌的演化和进展具有一定重要作用,并可能成为一个新的预后指标。

Expression and significance of fragile histidine triad in sporadic colonic cancer

Objective To investigate the expression of fragile histidine triad (FHIT) gene protien and it's possible relationship between FHIT expression and clinicopathological indices in sporadic colon cancer. Methods Using anti-human FHIT protein antibody of rabbit and citrate-micro wave-SP immunohistoehemical method, FHIT expressions were detected in the specimens of 74 cases of colon cancer,26 cases of normal tissuses and 26 cases of benign adenoma respectively. It's clinicopathologiecl indices (Dukes stage etc) and five years survival rate were analyzed. Rusults FHIT gene expressions were negative in l case of 26 cases(3.8%) of normal colonic tissues,in 7 of 26 cases(26.9%)of colonic adenoma tissues, in 35 of 74 cases(47.3%) of colon cancer tissues respectively.There were significant difference in the former three kings(P<0.05). FHIT protein low expressions were not associated with age,sex,tumor position and size,serum CEA level and histologic type. However, there were significant difference in the degree of differentiation,pathologic stage, lymph node metastasis, distant metastasis and 5 years survival rate(P<0.05). Conclusion Reduction of FHIT expressions may play an important role in the development and progression of the colon cancer. And it may become a new prognostic marker in colon cancer.