Cotinine determination by immunoassays may be influenced by other nicotine metabolites |
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Authors: | Georg Schepers Rüdiger-Alexander Walk |
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Institution: | (1) INBIFO Institut für biologische Forschung, Fuggerstrasse 3, D-5000 Köln 90, Federal Republik of Germany |
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Abstract: | Polyclonal rabbit anticotinine antiserum, which can be used for biomonitoring nicotine uptake by the determination of cotinine in body fluids, was checked by a competitive ELISA for its cross-reactivity with nine nicotine metabolites. The highest percentage of relative crossreactivity (about 30%) was observed with trans-3 -hydroxycotinine, a metabolite which is known to be excreted in 3-fold higher amounts than cotinine in the urine of human smokers. Therefore, it is possible that cotinine determinations performed by immunochemical methods — especially in urine — may yield overestimated cotinine concentrations. |
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Keywords: | Cotinine 3 -Hydroxycotinine" target="_blank">gif" alt="prime" align="BASELINE" BORDER="0">-Hydroxycotinine Cross reaction Enzyme-linked immunosorbent assay Biomonitoring |
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