鼻咽癌组织细胞凋亡的检测

目的 :探讨鼻咽癌组织放疗后病理改变及其临床意义。方法 :采用TdT酶介导的生物素化dutp缺口末端标记技术和HE染色 ,检测 30例鼻咽癌组织放疗前后的细胞凋亡率 ,并比较放疗前后的组织病理改变。结果 :鼻咽癌组织放疗后的细胞凋亡率明显高于放疗前 ;放疗后的癌细胞分化趋好 ,但到放疗中期时凋亡率有所下降。结论 :鼻咽癌放疗一方面可加速癌细胞的凋亡 ,另一方面可能促使增殖的癌细胞朝分化好的方向发展 ,从而达到治疗目的

Detection of radiation-induced apoptosis in nasopharyngeal carcinoma

Objective: To investigate the pathological changes after radiotherapy and its clinical significance of nasopharyngeal carcinoma (NPC).Method:Paraffin embedded tissue blocks obtained from 30 cases of NPC before and during radiotherapy were used in this study.Using terminal oxynucleotidyl transferase mediated dUTP biotin nick end labeling (TUNEL) and HE stainning,the spontaneous and radiation induced apoptotic rate (AR) were examined,and the pathological changes of NPC tissues before and after radiotherapy were compared.Result:The AR of radiation induced apoptosis was significant higher than that of spontaneous apoptosis,but the AR was decreasing and the differentiation of proliferatic tumor clonogehic cell getting better during radiotherapy of NPC.Conclusion:The process of apoptosis was accelerated and the differentiation of proliferatic tumor clonogehic cell getting better during radiotherapy of NPC.