流式细胞仪分析儿童急性淋巴细胞白血病免疫表型63例 |
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引用本文: | 何海龙,刘笑梅,王易,柴忆欢,王津媛,李祯萍,朱玲俐. 流式细胞仪分析儿童急性淋巴细胞白血病免疫表型63例[J]. 中国小儿血液与肿瘤杂志, 1999, 0(4) |
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作者姓名: | 何海龙 刘笑梅 王易 柴忆欢 王津媛 李祯萍 朱玲俐 |
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作者单位: | 苏州医学院附属儿童医院!邮编:215003(何海龙,王易,柴忆欢,王津媛,李祯萍,朱玲俐),苏州医学院附属儿童医院!邮编:215003(刘笑梅) |
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摘 要: | 为了了解儿童急性淋巴细胞白血病的免疫表型,应用一组单克隆抗体及流式细胞仪间接免疫荧光法对63例初治儿童急性淋巴细胞白血病(ALL)免疫分型:T淋巴细胞型、B淋巴细胞型、T/B混合型及裸核(Null型)ALL分别为41.3%、46%、9.5%、3.2%,髓系抗原(My)及CD(34)表达率为17.4%和28.5%,My+ALL中CD(34)表达为54.5%,显著高于My-ALL(23.3%)(P<0.01),T/B混合型ALL对My及CD(34)均有高表达,与T、B-ALL相比均有显著差异(P<0.01-0.05)
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关 键 词: | 急性淋巴细胞白血病 免疫 分型 流式细胞仪 |
IMMUNOPHEHOTYPING OF CHILDERN ACUTE LYMPHOBLASTIC LEUKEMIA |
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Abstract: | Use a Panel of mouclonal autibodies and flow cytometric i ndirect immunofluorescenctechnique. Sixty three cases of untreated childern acute lymphoblastic leukemia (ALL) Qereimmunophenot yed. Tere Were 41. 3 %、46 %、9.5 %、3.2 %of Tcell |B cell 、B cell .T1 B mixedand null ALL respectively. The myeloid associated antigens (My) and CD34 Were expressedin 17. 4% and 28. 5% . The expression rate of CD34in My+ ALL Was higher than My- ALL(p<0. 01 ) 。The expression rate of myeloid associated antigens and CD34in T/B mixed ALL washigher than T cell and B cell ALL(P<0. 05-0. 01 ). |
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