| 海南粗榧新碱衍生物 HH07A 对 DNA 聚合酶 I 活性的影响 |
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| 引用本文： | 叶玉梅,徐承熊.海南粗榧新碱衍生物 HH07A 对 DNA 聚合酶 I 活性的影响[J].药学学报,1997,32(5):337-339. |
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| 作者姓名： | 叶玉梅 徐承熊 |
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| 作者单位： | 中国医学科学院、中国协和医科大学药物研究所 |
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| 摘 要： | 用体外DNA聚合酶I作用下DNA合成的方法,研究HH07A的作用机制。结果表明,HH07A对DNA聚合酶I催化下的DNA合成有明显抑制作用,且在一定浓度范围内存在浓度依赖性。将药物与DNA模板及DNA聚合酶I分别预保温后,发现DNA模板活性无明显改变,而酶的活性则受到显著抑制。提示HH07A对DNA聚合酶I催化下DNA合成的抑制是通过HH07A与DNA聚合酶I直接作用而实现的。
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| 关 键 词： | 海南粗榧新碱衍生物 HH07A DNA聚合酶I 脱氧核糖核酸 抗癌药物 |
| 收稿时间： | 1996-06-20 |
EFFECT OF HH07A, A DERIVATIVE OF HAINANENSINE, ON DNA POLYMERASE I |
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| YM Ye and CX Xu.EFFECT OF HH07A, A DERIVATIVE OF HAINANENSINE, ON DNA POLYMERASE I[J].Acta Pharmaceutica Sinica,1997,32(5):337-339. |
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| Authors: | YM Ye and CX Xu |
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| Institution: | Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050. |
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| Abstract: | The effect of HH07A on DNA polymerase I in a cell free system was studied. The results showed that HH07A strongly inhibited the DNA synthesis catalyzed by DNA polymerase I in a dose dependent manner. Further results indicated that the DNA synthesis decreased significantly after DNA polymerase I was preincubated with HH07A. However, no change was found when DNA template was preincubated with HH07A. These results suggest that the inhibitory action of HH07A appeared to be on the enzyme molecules rather than on the DNA template. |
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| Keywords: | HH07A DNA polymerase I DNA Antineoplastic agent |
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