液相色谱法测定环境空气中的尼古丁

目的建立环境空气中气态尼古丁的液相色谱测定方法。方法被动式采样采集空气中的气态尼古丁,采样后滤膜经超声提取、溶剂萃取、氮吹后,用流动相溶解、C18反相色谱柱分离、紫外检测器检测,以保留时间定性,峰面积定量。结果检出限及测定下限分别为0.05μg/m3、0.16μg/m3;精密度为0.24%～2.62%;实际样品的加标回收率的范围在82.0%～107.2%;样品密封后,在干燥的室温状态下可以保存11天以上。结果该方法适宜于测定公共场所空气中尼古丁的浓度。

Determination of gaseous nicotine in ambient air by high performance liquid chromatography

Objective To establish the determination of gaseous nicotine in ambient air using high performance liquid chromatography.Methods The nicotine samples of ambient air were collected by passive air samples.The Sample film was sonicated,extracted,evaporated with nitrogen sweeping,and dissolved with the mobile phase.Nicotine was separated by C18 reversed-phase column,examined by ultraviolet detector,quantified by peak area and qualitatively measured by its retentive time.Results The limit of detection and the limit of quantification is 30ng/mL,101ng/mL,respectively.The precision is characterized by RSD in the rage 0.24%-2.62%.The average recovery of sample is in the rage of 82.0%-107.2%.The samples can be kept more than 11 days in the dry state of room temperature after sealing.Conclusion The method is suitable for the determination of nicotine concentration in public places air.