白藜芦醇对铅诱导小鼠脂质过氧化的拮抗作用

目的探讨白藜芦醇对铅暴露引起的氧化损伤的拮抗作用。方法铅染毒动物模型用含铅(醋酸铅0.5%)饮用水构建。60只健康雄性昆明种小鼠随机分为6组,每组10只:正常对照组,铅染毒组,铅染毒+白藜芦醇低、中、高剂量干预组,白藜芦醇组。检测小鼠血铅和组织铅含量及肝、肾组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性和丙二醛(MDA)的含量和抗氧化能力指数。结果与铅染毒组相比,白藜芦醇处理组肝、肾组织中SOD、CAT和GSH-Px活性均上升(P<0.01),MDA含量降低(P<0.01)。白藜芦醇使用剂量在800～1 200mg/kg时能够显著地提高机体抗氧化酶的活力,增强机体的抗氧化能力。铅染毒小鼠血浆、肝组织及肾组织中ORAC值均有一定程度的减小,给予白藜芦醇可以拮抗铅毒性引起的ORAC值的下降。结论白藜芦醇通过提高抗氧化酶活性,以减轻铅毒引起的脂质过氧化损伤,提高小鼠机体的抗氧化能力,而实现保护肝、肾组织的作用。

Antagonizing effect of resveratrol on the lipid peroxidative damage induced by lead in mice

Objective To explore the antagonizing effect of resveratrol on the lipid peroxidation and antioxidant enzymes induced by lead in mice's liver and kidney.Methods The lead poisoning mice model was established by drinking water,which contained lead acetate,60 Kunming male mice were randomly divided into 6 groups:control group,The lead poisoning model group,three lead groups treated by the low,medium and high dose of resveratrol,10 mice in each.The lead concentration and the content of MDA and activity of SOD,GSH-Px,CAT,and ORAC were measured in liver and kidney tissue and serum.Results Compared with poisoning model group,The activation of SOD,GSH-Px was increased in liver and kidney tissue of three lead groups treated with resveratrol.The activity of SOD,GSH-Px,CAT and the content of MDA were significantly different among the groups(P<0.01).the antagonizing dose of resveratrol was 800-1 200mg/kg,it can enhance enzymes activity.Conclusion Resveratrol may improve antioxidant enzymes activity and reduce lipid peroxidation in mice treated with lead in mice.