戊二醛交联提高胶原/壳聚糖真皮支架抗细胞收缩能力的研究

目的 应用交联处理的胶原支架,来减缓其降解过多、易收缩变形、机械性能不足等缺点.方法 采用冷冻-冻干法构建胶原/壳聚糖多孔支架,并通过戊二醛交联构建具有良好抗细胞收缩稳定性和细胞相容性的胶原支架.通过测定不同培养时间各类支架的尺寸变化,研究了戊二醛交联对支架抗细胞收缩能力的影响.结果 戊二醛交联后的胶原/壳聚糖支架未见明显收缩,其细胞活性也始终高于干热交联支架.结论 戊二醛能有效地提高胶原基支架的抗细胞收缩能力,并保持其良好的生物相容性.

Glutaraldehyde as a Crosslinker to Reduce the Cell-mediated Contraction of Collagen/Chitosan Scaffold

Objective To investigate the effect of collagen scaffold treated by cross-link to reduce biodegradability and low mechanical strengh. Methods Collagen/chitosan (Col/Chi) porous scaffolds were prepared by freeze-lyophilizing method and cross-linked with glutaraldehyde (GA). After seeded with human fibroblasts, the contraction behavior of the scaffolds was investigated as a function of culture time. Results No obvious contraction was observed for scaffolds cross-linked by GA. MTT assay revealed also that the fibroblast viability of the Col/Chi scaffold crosslinked by GA was larger than that of DHT treated one during the whole culture period. Conclusions All these results indicate that GA crosslinking is an easy but effective way to produce collagen based scaffold with retained cytocompatibility and enhanced ability to reduce the cell-mediated contraction.