心肌梗死患者细胞间黏附分子-1基因多态性研究

目的探讨汉族人群中细胞间黏附分子-1（ICAM-1）第4外显子＋12959G／A、第6外显子＋13848A／G基因多态性与心肌梗死（心梗）的相关性。方法提取165例心梗患者（包括急性心梗和陈旧性心梗）和199名健康对照者的DNA，采用序列特异性引物扩增法和聚合酶链反应-限制性片段长度多态性技术检测ICAM-1＋12959G／A、＋13848A／G基因多态性，同时测定血脂及血清超敏C-反应蛋白（hsCRP，采用定量酶联免疫吸附试验技术）水平。结果湖北地区汉族人群中存在＋13848A／G多态性，在心梗组中基因型分布为：AA型58．2％、AG型37．0％、GG型4．8％；对照组分别为45．7％、45．2％、9．0％，两组比较差异有统计学意义（P〈0．05）；基因型频率的相对风险分析发现，AA型患心梗的风险是GG型及AG型的1．651倍（OR=1．651，95％CI：1．089～2．504）。未检出＋12959G／A多态性。结论ICAM-1＋13848A／G基因多态性与湖北地区心梗易感性有关，＋13848A等位基因可能是心梗发病的重要易感基因。

Study on the intercellular adhesion molecule-1 polymorphisms in a Chinese population with myocardial infarction

Objective To investigate the association between intercellular adhesion molecule-1 (ICAM-1) gene + 12959G/A and + 13848A/G polymorphism and myocardial infarction (MI). Methods Polymerase chain reaction-sequence specific primers ( PCR-SSP) technology and PCR-restriction fragment length polymorphism(PCR-RFLP) were used for the detection of ICAM-1 genotypes in 165 patients with MI(including acute MI and old MI) and 199 healthy controls. Plasma lipid levels and hyper sensitive C reactive protein (quantitive ELISA method was used) levels were measured in all subjects. Results + 12959G/A polymorphism was not detected in our study. The frequencies of AA, AG and GG Genotypes of + 13848A/G were 58.2% and 45.7% ,37.0% among patients and 45.2%,4.8% and 9.0% among controls,respectively. There were statistically significant differences in the distributions of the genotype f requencies( P < 0.05) between two groups, and the relative risk suffered from MI of AA genotype was 1.651 times of the GG and GA genotype( OR = 1.651,95% CI: 1.089-2.504). Conclusion There was no + 12959G/A polymorphism found in Chinese people. The coding single nucleotide polymorphism + 13848A/G in the exon 6 of ICAM-1 gene was associated to MI and the allele A might serve as a risk factor for MI in Chinese.