Induction of chromosomal aberrations and spindle disturbances in Chinese hamster epithelial liver cells in culture by pyrene and benzo[a]pyrene quinones |
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Authors: | Sbrana Isabella; Puliti Aldamaria; Seidel Albrecht; Glatt Hansruedi; Turchi Gino |
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Institution: | 1Dipartimento di Scienze dell' Ambiente c del Temtorio, Universitá di Pisa Italy
2Institute of Toxicology, University of Mainz Mainz
3German Institute of Human Nutrition, Potsdam-Ren brll eke Germany
4Istituto di Mutagenesi e Differenziamento CNR, Pisa
5Joint Research Centre, European Centre for the Validation of Alternative Methods Ispra (VA), Italy |
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Abstract: | Regioisomers of pyrene and benzoa]pyrene quinones were testedfor their ability to induce structural and numerical aberrationsand spindle disturbance in Chinese hamster epithelial liver(CHEL) cells in culture. All quinones tested were clastogenicPyrene-1,8-quinone (P-1,8-Q) and benzoa]pyrene3,6quinone(BP-3,6-Q) induced strikingly high levels of triradials. Inaddition, dicentrics and ring chromosomes were very common inBP-3,6-Q-treated cultures. Isomers of these compounds, pyrene-1,6-quinone(P-1,6-Q) and benzoa]pyrene-1,6-quinone (BP-1,6-Q), inducedunobtrusive patterns of chromosomal aberrations. We suspectthat the P-1,8-Q and BP-3,6-Q moieties bound to the DNA werestill reactive, and formed crosslinks and/or underwent redoxcycling leading to high local concentrations of reactive oxygenspecies. In addition, P-1,8-Q and BP-3,6-Q induced c-mitoses,hyperdiploidies and polyploidies, in particular endoreduplications.These effects were not seen with the other two test compounds,or they were only detected at the highest concentrations used,which were strongly cytotoxic (c-mitoses with P-1,6-Q, polyploidieswith BP-1,6-Q).
6To whom correspondence should be addressed at: European Centre for the Validation of Alternative Methods (ECVAM), Joint Research Centre (JRC), TP58O, 121020 Ispra, Italy |
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