|
|||||
|
|
| 癌宁诱导体外人胃腺癌细胞SGC—7901凋亡及细胞周期分析 | |
| 引用本文： | 苏勉诚,吕晓英,李红坤,万斌,刘继林.癌宁诱导体外人胃腺癌细胞SGC—7901凋亡及细胞周期分析[J].中药材,2001,24(12):876-879. |
| 作者姓名： | 苏勉诚 吕晓英 李红坤 万斌 刘继林 |
| 作者单位： | 1. 广东省珠海市国家中药现代化工程技术研究中心, 2. 四川大学公卫学院, 3. 成都中医药大学, |
| 摘 要： | 目的：探讨中药复方－－癌宁诱导人胃腺癌细胞凋亡的作用。方法：采用DNA电泳和流式细胞术,对中药复方－－癌宁诱导人胃腺癌细胞凋亡进行了研究。结果：经癌宁处理后的人胃腺癌细胞出现DNA梯状图谱,流式细胞术检测其凋亡是分数高达43．5％,而对照组既无DNA梯状图谱,又无凋亡峰。细胞周期分析表明,经癌宁处理后,细胞的增殖指数为33．7％,无细胞周期特异性。结论：癌宁在体外对人胃腺癌细胞具有诱导凋亡作用。 |
| 关 键 词： | 中药复方 癌宁 胃腺癌细胞 细胞凋亡 细胞周期 |
| 修稿时间： | 2001年8月13日 |
Study on Apoptosis of Human Gastric Adenocarcinoma Cell SGC-7901 Induced by Aining in vitro and the Cell Cycle |
|
| Su Miancheng.Study on Apoptosis of Human Gastric Adenocarcinoma Cell SGC-7901 Induced by Aining in vitro and the Cell Cycle[J].Jorunal of Chinese Medicinal Materials,2001,24(12):876-879. | |
| Authors: | Su Miancheng |
| Institution: | National Engineering Research Center for Mordernization of TCM, Zhuhai 519020. |
| Abstract: | Objective: To study the inducing apoptosis effect of Aining on human gastric adenocarcinoma cell in vitro. Methods: Agarose gel electrophoresis (AGE) and flow cytometry (FMC) techniques were adopted to study the inducing apoptosis effect of Ainging on the human gastric adenocarcinoma cell. Results:The human gastric adenocarcinoma cell's DNA was digested into fragments which gave rise to a characteristic "DNA Ladder" on agarose gel electrophoresis, while the percentage of apoptotic cells was 43.5% . But the blank group had neither DNA ladder, nor apoptotic peak. Cell cycle analysis showed that the proliferation index was 33.7% after the human gastric adenocarcinoma cells treated with Aining, and there was no cell phase specialty. Conclusion:Aining can induce human gastric adenocarcoma cell apoptosis in vitro. |
| Keywords: | |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |