| 微波辐射非热效应诱导人肺癌A549细胞自噬的研究 |
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| 引用本文： | 曹慧玲,徐冶,刘晓冬,于洋,刘师兵,王晓军,孙路阳. 微波辐射非热效应诱导人肺癌A549细胞自噬的研究[J]. 实用肿瘤杂志, 2012, 27(3): 271-273 |
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| 作者姓名： | 曹慧玲 徐冶 刘晓冬 于洋 刘师兵 王晓军 孙路阳 |
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| 作者单位： | 吉林医药学院医学科研实验室,吉林吉林,132013 |
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| 基金项目： | 吉林省教育厅科学技术研究资助项目 |
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| 摘 要： | 目的探讨微波辐射非热效应对人肺癌A549细胞自噬的影响。方法用冰浴法制备微波辐射非热效应模型,剂量为100 W-12 mV/cm2、150 W-18 mV/cm2和200 W-21 mV/cm2,照射10分钟;24小时后,免疫印记法检测细胞自噬相关蛋白Beclin-1、LC3的变化,泛素化蛋白表达变化;间接免疫荧光法检测自噬标志蛋白LC3和p62的变化。结果微波辐射非热效应作用A549细胞,导致自噬相关蛋白Beclin-1表达增加,LC3和p62活化。结论微波辐射非热效应能够诱导人肺癌A549细胞发生自噬。
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| 关 键 词： | 肺肿瘤 微波/辐射效应 自噬 Beclin-1蛋白 免疫印迹法 |
Effect of autophagy on lung cancer cell line A549 by microwave radiation non-thermal effect |
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| Affiliation: | CAO Hui-ling,XU Ye,LIU Xiao-dong,et al(Medical Research Laboratory,Department of Histology ＆ Embryology,Jilin Medical College,Jilin,132013,China) |
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| Abstract: | Objective To investigate the effect of autophagy induced by microwave radiation non-thermal effect on human A549 cell line.Methods Ice bath was employed to establish microwave radiation non-thermal effect model,and dosages were 100 W-12 mV/cm2,150 W-18 mV/cm2 and 200 W-21 mV/cm2 for 10 minutes.After 24 hours,Western blot was used to detect the expression of Beclin-1,LC3 and ubiquitinated proteins.Indirect immunofluorescence was used to dectect the expression and location of the LC3 and p62 proteins.Results The non-thermal effect of microwave radiation on human A549 cells could increase the expression of Beclin-1,and induced activation of LC3 and p62.Conclusion The non-thermal effect of microwave radiation on human A549 cells could induce autophagy. |
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| Keywords: | lung neoplasms microwaves/radiation effects autophagy Beclin-1 protein immunoblotting |
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