CXCR1/CXCR2受体拮抗剂——G31P抑制前列腺癌转移的体内外实验研究 |
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引用本文: | 刘欣,李星云,王小丽,戴晓冬,李芳. CXCR1/CXCR2受体拮抗剂——G31P抑制前列腺癌转移的体内外实验研究[J]. 实用肿瘤杂志, 2012, 27(1): 73-76 |
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作者姓名: | 刘欣 李星云 王小丽 戴晓冬 李芳 |
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作者单位: | 1. 大连医科大学微生物学教研室,辽宁大连,116044 2. 大连医科大学寄生虫学教研室,辽宁大连,116044 3. 大连医科大学免疫学教研室,辽宁大连,116044 |
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摘 要: | 目的研究CXCR1/CXCR2受体拮抗剂——G31P对人前列腺癌PC-3细胞的体内外转移的抑制作用。方法采用细胞划痕实验研究G31P对PC-3细胞体外转移的抑制作用。通过建立体内的绿色荧光蛋白(GFP)标志人雄激素非依赖性前列腺癌PC-3细胞的裸鼠原位移植瘤模型,观察G31P对裸鼠前列腺癌原位移植瘤体内转移的影响。结果细胞划痕实验结果显示,G31P 100μg/L处理组作用细胞72小时的迁移率为(50.17±22.43)%(P<0.05,与对照组比较)。与对照组(100μL N.S)比较,G31P处理组(0.5 mg/kg)淋巴结(腰淋巴结、肠系膜淋巴结、远处淋巴结)转移略有减少,但差异无统计学意义;胰腺和肝部转移则明显减少(P<0.05)。免疫组织化学结果显示,与对照组比较,G31P处理组MMP-9(P<0.05)的表达明显减少。结论在体内外实验中G31P均能抑制人雄激素非依赖性前列腺癌PC-3细胞系的转移。
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关 键 词: | 前列腺肿瘤 肿瘤转移 受体,趋化因子 基因 点突变 |
Study on inhibition effect of G31P-a chemokine receptor CXCR1/2 antagonist on metastasis of human prostate cancer cells in vivo and in vitro |
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Affiliation: | LIU Xin,LI Xing-yun,WANG Xiao-li,et al(Department of Microbiology,Dalian Medical University,Dalian,116044,China) |
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Abstract: | Objective To investigate the inhibition effect of G31P on the metastasis of the prostate cancer PC-3 cells in vivo and in vitro. Methods The inhibition effect of G31P on metastasis of PC-3 cells was investigated by cell wound healing assay in vitro.The effect of G31P on metastasis of human prostate tumor of nude mice was observed by building the human androgen-independent prostate cancer PC-3(GFP-labeled) orthotopic transplantation tumor cells model in nude mice. Results The migration rate of the PC-3 cells of G31P 100 ng/mL treatment for 72 hours was(50.27±22.03)% by cell wound healing assay(P〈0.05,vs control group).Lymph nodes(lumbar lymph nodes,mesenteric lymph nodes,distant lymph node) metastasis of G31P treatment group(0.5 mg/kg) decreased slightly,which was not statistically significant;pancreas and liver metastasis were significantly reduced(P〈0.05,vs control group).MMP-9 expression of G31P treated group was significantly reduced by immunohistochemistry(P〈0.01) compared with the control group. Conclusion G31P could inhibit the metastasis of the PC-3 cell of the prostate cancer in vivo and in vitro. |
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Keywords: | prostatic neoplasms neoplasm metastasis receptors chemokine genes point mutation |
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