CXCR1/CXCR2受体拮抗剂——G31P抑制前列腺癌转移的体内外实验研究

目的研究CXCR1/CXCR2受体拮抗剂——G31P对人前列腺癌PC-3细胞的体内外转移的抑制作用。方法采用细胞划痕实验研究G31P对PC-3细胞体外转移的抑制作用。通过建立体内的绿色荧光蛋白(GFP)标志人雄激素非依赖性前列腺癌PC-3细胞的裸鼠原位移植瘤模型,观察G31P对裸鼠前列腺癌原位移植瘤体内转移的影响。结果细胞划痕实验结果显示,G31P 100μg/L处理组作用细胞72小时的迁移率为(50.17±22.43)%(P<0.05,与对照组比较)。与对照组(100μL N.S)比较,G31P处理组(0.5 mg/kg)淋巴结(腰淋巴结、肠系膜淋巴结、远处淋巴结)转移略有减少,但差异无统计学意义;胰腺和肝部转移则明显减少(P<0.05)。免疫组织化学结果显示,与对照组比较,G31P处理组MMP-9(P<0.05)的表达明显减少。结论在体内外实验中G31P均能抑制人雄激素非依赖性前列腺癌PC-3细胞系的转移。

Study on inhibition effect of G31P-a chemokine receptor CXCR1/2 antagonist on metastasis of human prostate cancer cells in vivo and in vitro

Objective To investigate the inhibition effect of G31P on the metastasis of the prostate cancer PC-3 cells in vivo and in vitro. Methods The inhibition effect of G31P on metastasis of PC-3 cells was investigated by cell wound healing assay in vitro.The effect of G31P on metastasis of human prostate tumor of nude mice was observed by building the human androgen-independent prostate cancer PC-3（GFP-labeled） orthotopic transplantation tumor cells model in nude mice. Results The migration rate of the PC-3 cells of G31P 100 ng/mL treatment for 72 hours was（50.27±22.03）% by cell wound healing assay（P〈0.05,vs control group）.Lymph nodes（lumbar lymph nodes,mesenteric lymph nodes,distant lymph node） metastasis of G31P treatment group（0.5 mg/kg） decreased slightly,which was not statistically significant;pancreas and liver metastasis were significantly reduced（P〈0.05,vs control group）.MMP-9 expression of G31P treated group was significantly reduced by immunohistochemistry（P〈0.01） compared with the control group. Conclusion G31P could inhibit the metastasis of the PC-3 cell of the prostate cancer in vivo and in vitro.