T淋巴细胞斑点试验检测在盆腔结核诊断中的应用

目的：探讨外周血和盆腔积液T淋巴细胞斑点试验（T-SPOT.TB）检测在盆腔结核诊断中的应用价值。方法采集28例确诊为盆腔结核患者及36例盆腔肿瘤患者（对照组）的外周血和盆腔积液，分离单个核细胞，采用T-SPOT.TB法检测特异度T淋巴细胞反应，同时对盆腔积液进行荧光定量PCR法检测，根据检测结果比较3种方法的灵敏度及特异度。结果外周血和盆腔积液T-SPOT.TB检测、盆腔积液荧光定量PCR法检测灵敏度分别60.71%，75.00%和28.57%，外周血和盆腔积液T-SPOT.TB检测2种方法比较，差异无统计学意义（χ2=1.310，P＞0.05），外周血和盆腔积液T-SPOT.TB检测与荧光定量PCR法均有统计学意义（χ2=7.292，P＜0.01和χ2=14.000，P＜0.001）。外周血和盆腔积液T-SPOT.TB检测、荧光定量PCR法检测特异度分别为91.97%、88.89%和86.11%。结论外周血和盆腔积液T-SPOT.TB检测对于盆腔结核的诊断有较好的应用价值。

Application of T-SPOT.TB assay in the diagnosis of pelvic tuberculosis

ObjectiveTo investigate the applicability of interferon gamma-released assay (T-SPOT.TB) for Mycobacterium tuberculosis (MTB) infections to the diagnosis of pelvic TB.MethodA total of 28 patients clinically diagnosed with pelvic tuberculosis and 36 patients with pelvic tumor were enrolled. The peripheral blood and pelvic effusions were detected by T-SPOT.TB, respectively. The pelvic effusions were also tested by real-time PCR for MTB. Then, sensitivities and specificities of the results were compared.ResultsThe sensitivity of T-SPOT.TB for the peripheral blood and pelvic effusions was 60.71% and 75.00%, respectively, but was 28.57% for the real-time PCR of pelvic effusions. There was no difference between T-SPOT. TB for the peripheral blood and that for pelvic effusions(χ2=1.310,P>0.05). However, the difference between T-SPOT.TB and real-time PCR was significant(χ2=7.292,P<0.01 andχ2=14.000,P<0.001). The specificity was 91.97%, 88.89% and 86.11%, respectively.ConclusionT-SPOT.TB performed on peripheral blood and pelvic effusions is of great value for diagnosis of pelvic tuberculosis.