石榴皮提取物对变形链球菌杀菌抑菌作用研究

目的分离提纯石榴皮多酚,研究其对变形链球菌的影响。方法石榴皮乙醇液经树脂吸附法分离提纯多酚,福林酚法检测石榴皮多酚纯度后用液体稀释法研究其抑菌作用和对细菌产酸的影响。结果分离提纯后得到纯度为83.02%的石榴皮多酚,最低抑菌浓度和最小杀菌浓度分别为0.5 g/L和1.0 g/L。随着药液浓度增加,细菌产酸能力减弱。结论石榴皮多酚具有能够较好抑制细菌的作用。

Extraction of pomegranate peel and its effect of antibacteria on streptococcus mutans

Objective To extract and purify pomegranate peel polyphenols, and to study its effect on streptococcus mutans. Methods Resin adsorption method was used to purify ethanolic extract to polyphenols. The purity of pomegranate peel polyphenols was tested by Foline-phenol method. Broth dilution test was used to study the antibacterial effort of polyphenols and its effect on bacterial acidoge-nicity. Results The purity of pomegranate peel polyphenols was 82. 03%. The minimal inhibitory concentration (MIC) was 0. 5 g/L and the minimal bactericidal concentration (MBC) was 1. 0 g/L. With the increasing concentrations of polyphenols, the acidogenicity capacity of bacteria was decreasing. Conclusion Pomegranate peel polyphenols has a potential effect on antibacteria.