| Nestin阳性细胞过表达DMP1对小鼠颌骨密度的影响 |
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| 引用本文： | 潘敏,孙瑶.Nestin阳性细胞过表达DMP1对小鼠颌骨密度的影响[J].口腔医学,2016(10):870-875. |
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| 作者姓名： | 潘敏 孙瑶 |
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| 作者单位： | 同济大学口腔医学院和附属口腔医院口腔种植科，上海牙组织修复与再生工程技术研究中心，上海 200072 |
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| 基金项目： | 国家自然基金(81300840、81470715)；2013年教育部博士点基金(20130072120024)；上海市卫生系统新优青项目(XYQ2013080)；高等学校博士学科点专项基金(20130072120024)；国家重点研发计划(2016YFC1102705) |
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| 摘 要： | 目的探讨Nestin阳性细胞中牙本质基质蛋白1(DMP1)的作用。方法 DMP1是牙齿和骨中重要的矿化蛋白,但是Nestin阳性细胞中DMP1的作用仍不清楚。我们构建了Nestin阳性细胞中过表达DMP1的转基因小鼠。以野生型(wild type,WT)小鼠为对照组,通过HE染色,Micro CT检测DMP1-Tg小鼠颌骨的变化,进一步通过TRAP染色检测DMP1-Tg小鼠颌骨破骨方面的变化。结果相对于WT小鼠,DMP1-Tg小鼠颌骨骨量下降;TRAP染色显示DMP1-Tg小鼠下颌骨破骨细胞增多。结论在下颌骨发育过程中,Nestin阳性细胞中过表达DMP1对骨形成发挥抑制作用。
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| 关 键 词： | Nestin 牙本质基质蛋白1 颌骨 小鼠 |
Influence of over expression of DMP1 in Nestin positive cells on the changes of jaw bone density in mice |
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| Abstract: | Objective To discuss the role of dental matrix protein 1( DMP1) in Nestin(+) cells. Methods DMP1 is an important mineralization protein of teeth and bones, but the role of DMP1 in Nestin(+) cells is still not clear. A transgenic mouse model was con?structed in which DMP1 over expressed in Nestin(+) cells. With wild mice as the control group, the DMP1-Tg mouse’ s jaw pheno?types by H&E staining and micro?CT were observed. Besides, the change of bone resorption in DMP1-Tg mouse by TRAP staining was also observed. Results Compared with WT mouse, the mandibular bone mass of DMP1-Tg mouse decreased;TRAP staining showed increased number of osteoclasts in DMP1-Tg mouse. Conclusion During the process of jaw development, the over expression of DMP1 in Nestin(+) cell can inhibit osteogenesis. |
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| Keywords: | Nestin dental matrix protein 1 jaw bone mouse |
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