慢病毒载体介导Golli-MBP基因过表达的体内实验初探

目的：通过过表达Golli?MBP基因尝试诱导口腔黏膜产生OLP病损，探讨Golli?MBP在OLP发病中的作用。方法：通过动物体内实验利用慢病毒载体系统包装诱导Golli?MBP过表达基因，将其注射于叙利亚金黄地鼠口腔黏膜下，肉眼观察黏膜改变，HE染色观察组织学改变，TUNEL法检测细胞凋亡，免疫组化法检测细胞增殖情况。结果：Golli?MBP未能诱导实验动物口腔黏膜产生肉眼可见的OLP病损变化，7天实验组上皮层细胞凋亡率与对照组无明显差异（ P＞0．05），14天实验组上皮层细胞凋亡率低于对照组（P＜0．05）。7天及14天实验组上皮层细胞增殖率低于对照组（P＜0．05）。结论：Golli?MBP 过表达能够抑制叙利亚金黄地鼠颊囊黏膜上皮细胞增殖和凋亡，但未能成功诱导其黏膜产生OLP病损。

A preliminary study of the lentiviral vector-mediated Golli-MBP gene overexpression in vivo

Objective: By overexpressing the oligodendrocyte lineage?myelin basic protein ( Golli? MBP ) gene to induce oral mucosa to produce oral lichen planus ( OLP ) lesions and explore the role of genes of Golli?MBP in the pathogenesis of OLP . Methods:By using lentiviral vectors to mediate Golli?MBP overexpression, which was injected under Syrian golden hamsters’ oral mucosa, we detected mucosal changes by visual observation , histological changes by HE staining, cell apoptosis by TUNEL assay, and cell prolif?eration by immunohistochemical method. Results:Golli?MBP failed to induce OLP lesions of experimental animals. There was no sig?nificant difference ( P>0.05) of apoptosis rate in experimental groups and the control groups in the epithelia lamina on the 7th day and the apoptosis rate on 14th day in the experimental groups was lower than that of the control groups, the difference was statistically sig?nificant ( P<0.05) . Cortical cell proliferation rate on the 7th day and 14th day in the experimental groups was lower than that of the control groups and the difference was statistically significant ( P<0.05) . Conclusions:Golli?MBP overexpression can suppress the pro?liferation and apoptosis of Syrian golden hamster cheek pouch mucosal epithelial cells. However, we failed to induce it to produce OLP lesions.