| miR-223调控人牙周膜成纤维细胞中RANKL表达的功能初探 |
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| 引用本文： | 杨迷芳,王珏,马兰,谢理哲,于金华.miR-223调控人牙周膜成纤维细胞中RANKL表达的功能初探[J].口腔生物医学,2016(4):183-186. |
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| 作者姓名： | 杨迷芳 王珏 马兰 谢理哲 于金华 |
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| 作者单位： | 1. 南京医科大学口腔疾病研究江苏省重点实验室,江苏 南京,210029;2. 南京医科大学口腔疾病研究江苏省重点实验室，江苏 南京210029; 南京医科大学附属口腔医院儿童口腔预防科，江苏 南京210029 |
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| 基金项目： | 江苏高校优势学科建设工程资助项目(2014-37) |
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| 摘 要： | 目的：探讨miR?223对人牙周膜成纤维细胞中核因子?κB受体活化因子配体（ RANKL）的调控作用。方法：构建过表达miR?223的人牙周膜成纤维细胞，Western blot及实时定量RT?PCR检测人牙周膜成纤维细胞内RANKL和骨保护素（ OPG）的表达，计算RANKL／OPG比值的改变。结果：过表达miR?223的人牙周膜成纤维细胞，RANKL的表达明显下调，RANKL／OPG比值下降。结论：miR?223可调控RANKL的表达，破坏RANKL／OPG比例的平衡，改变破骨细胞的微环境，影响破骨细胞分化。
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| 关 键 词： | 微小RNA-223 人牙周膜成纤维细胞 核因子-κB受体活化因子配体 骨保护素 |
Investigation of miR-223 on the expression of RANKL in human periodontal ligament fibroblasts cells |
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| Abstract: | Objective: To investigate the role of miR?223 on the expression of receptor activator for nuclear factor?κB ligand ( RANKL) in human periodontal ligament fibroblasts cells ( HPDLFs) . Methods:The HPDLFs over expressed with miR?223 was con?structed, the expression of RANKL and osteoprotegerin ( OPG) were detected by Western blot and real?time RT?PCR, and the change of RANKL/OPG ratio was calculated. Results:The RANKL expression was down?regulated in the HPDLFs with miR?223 over expressed and the RANKL/OPG ratio was decreased. Conclusions:MiR?223 could disrupt the homeostasis of RANKL/OPG, destroy the micro?environment of osteoclast differentiation. MiR?223 may play a regulatory role in osteoclast differentiation. |
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| Keywords: | miR-223 human periodontal ligament fibroblasts cells RANKL OPG |
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