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2种方法使用生物活性玻璃脱敏剂对牙本质小管封闭作用的场发射扫描电镜观察
引用本文:马骞,罗瑞,王婕,王天达,陈亚明. 2种方法使用生物活性玻璃脱敏剂对牙本质小管封闭作用的场发射扫描电镜观察[J]. 口腔医学, 2016, 0(11): 997-1001
作者姓名:马骞  罗瑞  王婕  王天达  陈亚明
作者单位:南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院综合科,江苏南京 210029
基金项目:南京市科技计划项目(201503062),江苏高校优势学科建设工程资助项目(2014-37);南京医科大学口腔医学院横向合作项目(22011001)
摘    要:目的使用场发射扫描电镜观察涂抹法和透明压膜托盘法使用生物活性玻璃脱敏剂在短时间内对牙本质小管的封闭效果,为增强生物活性玻璃的脱敏疗效提供新思路。方法制取56颗离体牙的牙本质片,6%柠檬酸脱矿后按处理方法和处理时间不同随机分为7组,涂抹法1 d组(A1组),涂抹法3 d组(A2组),涂抹法7 d组(A3组),压膜法1 d组(B1组),压膜法3 d组(B2组),压膜法7 d组(B3组),空白对照组。通过FESEM观察比较各组牙本质小管封闭效果。结果涂抹法和透明压膜托盘法使用生物活性玻璃脱敏剂后均能很好的封闭牙本质小管,牙本质小管内均可见明显矿化物沉积,A1、A2、A3、B1、B2、B3组牙本质小管的暴露率分别为(6.29±1.18)%、(3.40±0.84)%、(1.23±0.43)%、(5.31±0.97)%、(1.40±0.30)%、(0.36±0.15)%,A2组>B2组,A3组>B3组,差异有统计学意义;空白对照组牙本质小管口开放,周界清晰。结论透明压膜托盘法在短时间内可以增强生物活性玻璃对敏感牙本质小管的封闭效果,与涂抹法相比,封闭效果有提高,差异有统计学意义。

关 键 词:生物活性玻璃  再矿化  透明压膜托盘法  场发射扫描电镜

Scanning electron microscope observation of occlusion effect on dentinal tubule by two application methods of bioglass
Abstract:Objective The study was carried out to evaluate In Vitro occlusion effect on dentinal tubule orifice by smearing or trans?parent pressure?swage application of bioactive glass desensitizer using FESEM. To provide rationale and evidence for strengthening effi?cacy of bioactive glass desensitizing treatment. Methods 56 dentin slices were prepared from extracted teeth and treated with 6% citric acid solution to demineralization, then dentin slices were randomly divided into 7 groups according to different treating methods and du?rations:smearing 1day group (A1), smearing 3 day group (A2), smearing 7 day group (A3), pressure?swage 1day group (B1), pressure?swage 3day group ( B2) , pressure?swage 7day group ( B3) and negative control group. Occlusion effect was observed in den?tinal tubule orifice in each group via Field emission scanning electron microscope ( FESEM) . Result FESEM results indicated that dentinal tubule was well occluded after smearing or transparent pressure?swage application of bioactive glass desensitizer. Apparent min?eral deposition was observed in dentinal tubule. Dentinal tubule exposure rates were A1(6.29±1.18)%、A2(3.40±0.84)%、A3(1.23± 0.43)% 1、3、7 days after smearing method. Dentinal tubule exposure rates were B1(5.31±0.97)%、B2(1.4±0.30)%、B3(0.36± 0.15)% 1、3、7 days after pressure?swage method. The difference of dentinal tubule exposure between A1and B1 was not statistically significant (p value=0.42). While differences of exposure in A2/B2 and A3/B3 were statistically significant (p value=0.007 and 0.013). In negative control group, dentinal tubule was open with clear border and dentinal tubule exposure rate was (19.11±1.27)%. Conclusion transparent pressure?swage method can improve occlusion effect of bioactive glass desensitizer in a short period of time. Compared with smearing method the improvement is significant.
Keywords:bioactive glass  remineralization  transparent pressure-swage method  field emission scanning electron microscope
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