| 正常人外周血淋巴细胞 IL—2受体免疫放射测定 |
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| 引用本文： | 殷志伟,杨惠斌,冯树一,步鸣,张学光,茅子均. 正常人外周血淋巴细胞 IL—2受体免疫放射测定[J]. 苏州大学学报(自然科学版), 1991, 0(4) |
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| 作者姓名： | 殷志伟 杨惠斌 冯树一 步鸣 张学光 茅子均 |
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| 作者单位： | 苏州医学院病理生理学教研室(殷志伟，杨惠斌，冯树一，步鸣，张学光)，苏州医学院病理生理学教研室(茅子均) |
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| 基金项目： | 江苏省科学技术委员会资助课题 |
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| 摘 要： | 采用~(125)I 标记小鼠抗人IL—2受体(P55)的抗Tac(CD_(25))单克隆抗体,成功地建立了人IL—2受体的免疫放射分析法,动态观察了人外周血淋巴细胞经PHA 刺激24、48和72h 后IL—2受体表达的时间曲线,通过Scatchard 作图分析表明~(125)I—抗Tac 单克隆抗体与PHA 活化的上述三个时间点的T 淋巴细胞的最大结合容量(B_(max))分别为43000位点/细胞、54000位点/细胞和61000位点/细胞。本研究为临床IL—2受体检测提供一种简便的免疫放射测定法。
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| 关 键 词： | 人淋巴细胞 IL-2受体 免疫放射分析 |
Immunorradiometric Assay of Normal Human Peripheral Blood Lymphocyte Interleukin 2 Receptor |
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| Yin Zhiwei,et al. Immunorradiometric Assay of Normal Human Peripheral Blood Lymphocyte Interleukin 2 Receptor[J]. Suzhou University Journal of Medical Science, 1991, 0(4) |
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| Authors: | Yin Zhiwei et al |
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| Abstract: | The regulation of IL-2R expression plays an important role in lymphocyteproliferation.Using (125)~I labelled anti-Tac,which is a mouse-antihuman IL-2R(P55)monclonal antibody,we had established the immunoradiometric assay of IL-2R andstudied the time course(24,48 and 72 hours)of human IL-2R expression after PHA stimulation.The results showed that the B_(max) of (125)~I labelled anti-Tac molecules bound toT cells at the intervals mentioned above after PHA stimulation was 43000(24 hours),54000(48 hours)or 61000(72 hours)per cell respectively. |
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| Keywords: | lymphocytes interleukin 2 recepter (IL-2R) immunoradiometric assay |
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