HLA—A,B血清学分型与DNA分型的比较

目的：比较１３６例样本ＨＬＡ－Ａ,Ｂ血清学分型与ＤＮＡ分型结果。方法：应用聚合酶链反应－序列特异性引物（ＰＣＲＳＳＰ）技术,设计３８对引物,检测常见的ＨＬＡ－Ａ,Ｂ基因。结果：１３６例样本中有６０例血清学分型与ＤＮＡ分型结构不符合（４４．１％）;ＨＬＡ－Ａ特异性血清学分型错误率在纯合子中分别占２３．３％与９．４％（共１１．２％）,在ＨＬＡ－Ｂ中分别占４７．４％与１８．４％（共２０．６％）;ＨＬＡ－Ａ特异性血清学分型假阴性６．６％,假阳性２．５％,错误指认特异性２．１％,ＨＬＡ－Ｂ错异性则分别为６．７％,３．６％,１０．３％。结论：ＩＬＡ－Ａ纯合子血清学分型错误率明显高于杂合子（Ｐ〈０．０５）;ＨＬＡ－Ｂ纯合子血清学分型错误率也明显高于杂合子（Ｐ〈０．００５）;ＨＬＡ－Ｂ特异性血清学分型错误率显著高于ＨＬＡ－Ａ特

Comparison of serologicaland DNA typing for HLA-A,B

Objective:To compare the result of HLA A,B serological typing with DNA typing in 136 samples Methods:All the HLA A,B frequent alleles were typed by PCR SSP with 38 pairs of primers Results:60 of 136 samples(44 1%) showed discrepancies between serological and DNA typing;The error rate of HLA A serological typing was 23 3% in the homozygotes and 9 4% in the heterozygotes(total 11 2%) and at the HLA B was 47 4% and 18 4%(total 20 6%);False positive,false negative and misassignment of the HLA A serological typing were 6 6%,2 5% and 2 1% respectively,of the HLA B were 6 7%,3 6% and 10 3% respectively Conclusion:Error rate of HLA A serological typing in the homozygotes was significantly higher than in the heterozygotes(P<0 050) ;Error rate of HLA B serological typing in the homozygotes was also higher than in the heterozygotes(P<0 005);Error rate of HLA B serological typing was higher than that of HLA A (P<0 005)