| 用聚合酶链反应检测人肝细胞癌乙型肝炎病毒DNA和丙型肝炎病毒RNA |
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| 引用本文： | 张惠忠,唐漾波,陆献瑜. 用聚合酶链反应检测人肝细胞癌乙型肝炎病毒DNA和丙型肝炎病毒RNA[J]. 中华病理学杂志, 1996, 0(2) |
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| 作者姓名： | 张惠忠 唐漾波 陆献瑜 |
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| 作者单位： | 中山医科大学肿瘤医院病理科，广州第八人民医院肝病研究所 |
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| 摘 要： | 为研究乙型肝炎病毒ＤＮＡ（ＨＢＶＤＮＡ）和丙型肝炎病毒ＲＮＡ（ＨＣＶＤＮＡ）与肝细胞癌的关系，用聚合酶链反应（ＰＣＲ）和巢式ＰＣＲ（ｎｅｓｔｅｄ－ＰＣＲ）分别检测４２例肝肿瘤组织中ＨＢＶＤＮＡ和ＨＣＶＲＮＡ。结果：１例胆管细胞癌组织ＨＢＶＤＮＡ和ＨＣＶＲＮＡ均阳性，１例胆管囊腺瘤ＨＢＶＤＮＡ阳性。４０例肝细胞癌组织中，单纯ＨＢＶＤＮＡ阳性１９例，单纯ＨＣＶＲＮＡ阳性３例，二者均阳性１０例。ＨＢＶＤＮＡ阳性率７２．５％，ＨＣＶＲＮＡ阳性率３２．５％。ＨＢＶＤＮＡ和ＨＣＶＲＮＡ感染与肝癌组织学分型无关；且肝细胞癌中ＨＣＶ感染与ＨＢＶ未见相关。结果提示，我国ＨＢＶ感染仍是引起肝细胞癌的主要原因。但由于肝细胞癌患者中ＨＣＶ的感染率也较高，且有上升趋势，因此ＨＣＶ可能也是肝细胞癌发生的重要原因之一。
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| 关 键 词： | 肝肿瘤 脱氧核糖核酸.病毒 核糖核酸.病毒 聚合酶链反应 |
DetectionofhepatitisBvirusDNAandhepatitisCvirusRNAinhumanhepatocelularcarcinomabypolymerasechainreaction |
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| ZhangHuizhong,TangYangbo,LuXianyu. DetectionofhepatitisBvirusDNAandhepatitisCvirusRNAinhumanhepatocelularcarcinomabypolymerasechainreaction[J]. Chinese Journal of Pathology, 1996, 0(2) |
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| Authors: | ZhangHuizhong TangYangbo LuXianyu |
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| Affiliation: | ZhangHuizhong,TangYangbo,LuXianyu.DepartmentofPathology,Sunyat-senUniversityofMedicalSciencesCancerCenter,Guangzhou510060 |
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| Abstract: | InordertostudytherelationshipbetweenhepatitisBvirusDNA(HBVDNA),hep-atitisCvirusRNA(HCVRNA)andlivercelcarcinoma,HBVDNAandHCVRNAfromthetumortissueof42livercelcarcinomacaseswerestudiedbypolymerasechainreaction(PCR)andnested-PCRrespectively.Theresultswereasfolows:onecaseofcholangiocarcinomawaspositiveforbothHBVDNAandHCVRNA,andonecaseofbiliarycystadenomapositiveonlyforHBVDNA.Amongthe40casesofhepatocelularcarcinoma(HCC),29werepositiveforHBVDNAand13positiveforHCVRNA.TherewasnorelationshipobtainedbetweenHBVorHCVinfectionandthehistologicaltypesofHCC,andHCVinfectioninHCCwasnotinterrelatedwithHBV.It'sconsideredthatal-thoughHBVisstiltobetheleadingcauseofHCCinChina,HCVmayplayanimportantroleinhep-aticcarcinogenesisbecauseofitshighpositiverateandincreasingincidence. |
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| Keywords: | LiverneoplasmsDNA viralRNA viralPolymeraseChianreaction |
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