| 高效液相色谱法测定避孕套中苯佐卡因的含量 |
| |
| 引用本文： | 母瑞红,柯林楠,周红娇. 高效液相色谱法测定避孕套中苯佐卡因的含量[J]. 中国医疗器械信息, 2010, 16(12): 37-38 |
| |
| 作者姓名： | 母瑞红 柯林楠 周红娇 |
| |
| 作者单位： | [1]中国药品生物制品检定所,北京100050 [2]国家药品食品监督管理局医疗器械标准管理中心,北京100050 [3]云南省医疗器械检验所,昆明650043 |
| |
| 摘 要： | 目的：采用高效液相色谱法（HPLC）测定避孕套中苯佐卡因（benzocaine）的含量。材料与方法：采用Polaris C18-A（5μm 250×4.6mm）色谱柱,用乙腈-水60：40作为流动相,检测波长为265nm,流速：1.0ml＊min-1,进样量10μl。结果：苯佐卡因在98.5μg/ml~492.5μg/ml范围内呈良好的线性关系（相关系数R2=0.9998）,精密度（RSD）0.7%,平均回收率为102.4%。结论：该方法操作简便可行,适用于含苯佐卡因避孕套中苯佐卡因含量的质量控制。
|
| 关 键 词： | 苯佐卡因 避孕套 高效液相色谱法（HPLC） |
Determination of Benzocaine in Condom by HPLC |
| |
| MU Rui-hong,KE Lin-nan,ZHOU Hong-jiao. Determination of Benzocaine in Condom by HPLC[J]. China Medical Devices Information, 2010, 16(12): 37-38 |
| |
| Authors: | MU Rui-hong KE Lin-nan ZHOU Hong-jiao |
| |
| Affiliation: | National Institute for the Control of Pharmaceutical ＆ Biological Products(Beijing 100050) 2 Center for Medical Device Standardization Administration,SFDA(Beijing 100050) 3 Yunnan Institute for the Control of Medical Device(Kunming 650043) |
| |
| Abstract: | Objective： To establish a HPLC method for determination of benzocaine in condom.Methods： A Polaris C18-A column（5μm 250×4.6mm） was used. The mobile phase was composed of acetonitrile-water （60：40）and its flow rate was 1.0ml/min. The detection wavelength was at 265nm.Results： The calibration curve was linear in the range of 98.5μg/ml to 492.5μg/ml（R2=0.9998）.The average recovery was 102.4%,RSD=0.7%.Conclusion： This method is simple and accurate.It can be applied for determination of benzocaine in condom. |
| |
| Keywords: | benzocaine condom HPLC |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
