| 组织因子单克隆抗体及对照抗体的制备、纯化和鉴定 |
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| 引用本文： | 亓翠玲,郭四美,叶杰,周秦,钟建国,王丽京.组织因子单克隆抗体及对照抗体的制备、纯化和鉴定[J].中国医疗前沿,2012(11):24-25. |
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| 作者姓名： | 亓翠玲 郭四美 叶杰 周秦 钟建国 王丽京 |
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| 作者单位： | 广东药学院血管生物学研究所 |
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| 基金项目： | 国家自然基金(编号:30900764);广东省医学科研基金(编号:A2011299) |
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| 摘 要： | 目的纯化组织因子(TF)单克隆抗体及对照抗体。方法给小鼠腹腔注射杂交瘤8A3和9E10细胞,产生的腹水应用凝胶过滤层析法和Protein A亲和层析法纯化组织因子抗体及对照抗体,并用SDS-PAGE和BCA法检测纯化后单克隆抗体的纯度及蛋白浓度。结果单抗纯化后电泳重链和轻链都呈一条带,TF抗体和对照抗体的最终浓度分别为0.7726mg/ml和0.8592mg/ml。结论应用层析法和凝胶过滤和Protein A亲和分离层析法纯化抗体,能够得到纯度较高的单克隆抗体。
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| 关 键 词： | 组织因子 单克隆抗体 亲和层析 纯化 |
Purification and identification of tissue factor monoclonal antibodies |
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| Institution: | QI Cui-ling,GUO Si-mei,YE Jie,et al.Vascular Biology Research Institute,Guangdong Pharmaceutical University,Guangzhou 510006,China |
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| Abstract: | Objective To purify and identify tissue factor monoclonal antibodies.Methods Firstly,8A3 and 9E10 cells were injected into abdominal cavity of BALB/c mice;then make affinity chromatography column with protein A-Sepharose CL-4B to purify tissue factor(TF) monoclonal antibodies.The purified specific TF monoclonal antibody was identificated and detected by SDS-PAGE and BCA.Results Purified TF antibody was electrophoresised and could show single band.The concentration of purified TF monoclonal antibody and control antibody respectively were 0.986mg/ml and 3.57mg/ml.Conclusion Affinity chromatography through protein A-Sepharose CL-4B can purify monoclonal antibody effectively. |
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| Keywords: | Tissue Factor Purification Affinity chromatography Monoclonal antibody |
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