| HPLC法测定复方利血平中氢氯噻嗪的含量 |
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| 引用本文： | 张华,吴勇. HPLC法测定复方利血平中氢氯噻嗪的含量[J]. 亚太传统医药, 2012, 8(8): 24-25 |
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| 作者姓名： | 张华 吴勇 |
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| 作者单位： | 1. 马鞍山十七冶医院,安徽马鞍山,243000
2. 马鞍山市药品检验所,安徽马鞍山,243000 |
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| 摘 要： | 目的:建立HPLC法测定复方利血平片中氢氯噻嗪含量的方法。方法:采用SunfireTM C18色谱柱;甲醇-0.1%己烷磺酸钠溶液(磷酸调节pH=3.0±0.1)(20:70)为流动相;流速为1.0mL.min-1;检测波长为308nm。结果:氢氯噻嗪的线性范围:14.11～70.55μg/mL(r=0.999 9),平均回收率为100.31%,RSD为1.11(n=4)。结论:本方法操作简单、快速、准确,可用于复方利血平片中氢氯噻嗪含量测定。
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| 关 键 词： | 高效液相 氢氯噻嗪 复方利血平片 |
Determination of Hydrochlorothiazide in Compound Reserpin Tablets by HPLC |
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| Zhang Hua , Wu Yong. Determination of Hydrochlorothiazide in Compound Reserpin Tablets by HPLC[J]. Asia-Pacific Traditional Medicine, 2012, 8(8): 24-25 |
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| Authors: | Zhang Hua Wu Yong |
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| Affiliation: | 1.Maanshan Hospital of 17th Metallurgical Construction Compang,AnHui 243000,China; 2.Maanshan institute for drug control AnHui 243000,China) |
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| Abstract: | Objective:A method was established for the determination of the content of hydrochlorothiazide in compound reserpin tablets.Methods:The separation was preformed on SunfireTM C18 column,using methanol-sodium hexanesulfonate(adjust PH to PH=3.0±0.1 by) phosphoric acid)(30:70),the flow rate was 1.0mL·min-1 and detecting wave length was 308nm.Results:the linear range of hydrochlorothiazide was 14.11~70.55μg(r=0.999 9) and average recovery was 100.31%,RSD=1.11%(n=4).Conclusion:The metheod was simple,fast and accurate,It can be used for determination of the content of hydrochlorothiazide in compound reserpin tablets. |
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| Keywords: | HPLC Hydrochlorothiazide Compound Reserpin Tablets |
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