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| 合格献血者血浆HBV残留情况分析 | |
| 引用本文： | 李平,刘勇,李翠萍,毕永春,张葵,周乙华.合格献血者血浆HBV残留情况分析[J].临床输血与检验,2008,10(4):293-296. |
| 作者姓名： | 李平 刘勇 李翠萍 毕永春 张葵 周乙华 |
| 作者单位： | 1. 江苏省南京大学医学院附属鼓楼医院输血科,210008 2. 210008,江苏省南京大学医学院附属鼓楼医院检验科;南京大学江苏省医学分子技术重点实验室 3. 江苏省南京大学医学院附属鼓楼医院检验科,210008 4. 210008,江苏省南京大学医学院附属鼓楼医院检验科;210008,江苏省南京大学医学院附属鼓楼医院感染科;南京大学江苏省医学分子技术重点实验室 |
| 摘 要： | 目的调查合格献血者血浆残留HBV的阳性率,评估输血后HBV感染风险。方法收集945份合格献血者的血浆,采用ELISA法检测HBV核心抗体（抗-HBc）;提取血浆DNA,采用巢式PCR方法检测HBV DNA。结果血浆标本以1∶5稀释后抗-HBc阳性率为19.68%,而1∶50稀释后阳性率则为2.33%。共检测出HBV DNA阳性标本3例,其中1例抗-HBc阳性,另外2例抗-HBc为阴性。合格献血者中隐匿性HBV感染率约为0.32%。结论我国合格献血者标本中仍有部分血浆有HBV残留,抗-HBc阳性标本中HBV DNA阳性率较抗-HBc阴性标本高,应引起重视。 |
| 关 键 词： | 合格献血者 乙型肝炎病毒核酸 抗-HBc 巢式PCR |
Prevalence of Occult HBV Infection in Accepted Blood Donors |
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| Institution: | LI Ping,LIU Yong, LI Cui-Ping,et al. (Departments of Transfusion Medicine in Gulou Hospital Affilicatied Nanjing University Medical College, Nanjing 210008) |
| Abstract: | Objective To investigate the positive rate of the occult HBV infection in accepted blood donors and to evaluate tbe risk of transfusion-transmitted HBV. Methods The 945 plasma specimens from blood donors were detected for anti-HBc by ELISA and HBV DNA by nested PCR. Results The positive rate of the anti-HBc was 19.68% when the specimens were 5-fold diluted, while the positive rate was 2. 330/00 when the specimens were 50-fold diluted. HBV DNA was detected in 3 of 945 subjects （0. 32% ）. Of the three donors, one was positive for anti-HBc after 50-fold dilution, and the other two were negative for anti-HBc. Conclusion It should arouse our attention that there is occult infection of HBV in accepted blood donors. The positive rate of HBV DNA in donors with anti-HBc was higher than that in anti-HBc negative donors. |
| Keywords: | Accepted blood donors HBV DNA Anti-HBc Nested PCR |
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