| 特异性抑制Aqp1基因对K562细胞生长的抑制作用 |
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| 引用本文： | 刘坚,孙朝晖,石玉玲,王蜀燕,危敏.特异性抑制Aqp1基因对K562细胞生长的抑制作用[J].中国实验血液学杂志,2010,18(1):40-44. |
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| 作者姓名： | 刘坚 孙朝晖 石玉玲 王蜀燕 危敏 |
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| 作者单位： | 1. 广州军区广州总医院医务部检验科,广东广州,510010
2. 广州南方医科大学基因工程研究所,广东广州,510515 |
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| 基金项目： | 广东省自然科学基金资助,编号8151001002000026 |
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| 摘 要： | 本研究探讨靶向aqp1(aquaporin 1)基因的siRNA对K562细胞生长的抑制作用。设计siRNA,转染K562细胞,在相差显微镜观察细胞形态变化;用MTT法观察aqp1-siRNA对K562细胞活力的影响;使用流式细胞仪、DNA片段化分析K562细胞凋亡;用RT-PCR半定量检测转染前后aqp1基因表达。结果表明:苏木素-伊红染色后,细胞呈现明显的凋亡形态;转染24小时后aqp1-siRNAs对K562细胞的活性有显著抑制作用;aqp1-siRNA转染K562细胞后可见明显的凋亡峰,24、48和72小时的细胞凋亡率持续上升,分别为24.2%、36.1%和42.9%;48小时后aqp1-siRNA组出现明显的DNA凋亡"梯形电泳带";aqp1基因的表达水平在aqp1-siRNA转染后24、48和72小时,分别减少了33%、46%和57%。结论:aqp1-siRNA能够抑制K562细胞的增殖,诱导其凋亡,可作为治疗恶性肿瘤的新靶点。
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| 关 键 词： | 水通道蛋白I K562细胞 RNA干扰 细胞凋亡 |
Inhibitory Effect of siRNA Targeting Gene Aqp1 on K562 Cells |
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| LIU Jian,SUN Zhao-Hui,SHI Yu-Ling,WANG Shu-Yan,WEI Min.Inhibitory Effect of siRNA Targeting Gene Aqp1 on K562 Cells[J].Journal of Experimental Hematology,2010,18(1):40-44. |
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| Authors: | LIU Jian SUN Zhao-Hui SHI Yu-Ling WANG Shu-Yan WEI Min |
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| Institution: | ( Department of Medical Administration, l Department of Clinical Laboratory Examination, General Hospital of Guangzhou Military Area, Guangzhou 510010, Guangdong Province, China; 21nstitute of Genetic Engineering, Southern Medical University, Guangzhou 510515, Guangdong Province, China) |
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| Abstract: | The objective of this study was to establish an RNAi approach that can specifically target aqp1 gene sequence in vitro,and to assess the inhibitory effect of this siRNA on K562 cells.The siRNA targeting aqp1-mRNA was designed and transfected into K562 cells by using LipofectamineTM 2000 reagent.Phase-contrast microscopy was used to analyze morphology changes of K562 cells.Cell viability was determined by MTT assay,and flow cytometry and DNA ladder analysis were carried out to identify siRNA-induced apoptosi... |
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| Keywords: | gene aqp1 K562 cell RNA interference apoptosis |
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