大豆异黄酮和壬基酚对雄性大鼠生殖系统的联合作用

目的]探讨大豆异黄酮、壬基酚联合孕期暴露对雄性子代大鼠生殖系统的危害。方法]SD大鼠同笼交配获取40只孕鼠。将孕鼠随机分为5组:对照组(A)、200mg/kg壬基酚组(B)、200mg/kg壬基酚 50mg/kg大豆异黄酮组(C)、200mg/kg壬基酚 150mg/kg大豆异黄酮组(D)、200mg/kg壬基酚 450mg/kg大豆异黄酮组(E)。孕期灌胃染毒,对其子一代雄鼠90天龄剖杀检测其精子数量、质量,睾丸组织重量、酶活性等。结果]与A组比较,B组睾丸重量和睾丸系数、精子活动度、附睾尾精子计数、每日精子生成量、睾丸组织碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)和血清睾酮(TT)水平显著下降,壬基酚与各剂量大豆异黄酮联合作用组睾丸重量、精子活动度、附睾尾精子计数、睾丸每日精子生成量、ALP、TT水平均显著下降;与B组比较,C组的睾丸重量、睾丸系数、睾丸每日精子生成量、ALP、LDH水平均显著升高,E组睾丸重量、睾丸系数、每日精子生成量、ALP、LDH显著升高而平均窝活产只数、精子活动度睾酮水平表现出下降的趋势。结论]50mg/kg大豆异黄酮对壬基酚所致雄性生殖系统危害似具有拮抗作用。450mg/kg的大豆异黄酮对壬基酚的雄性生殖系统危害似表现出相加作用。

The Joint Action of Nonylphenol and Soy Isoflavone on the Reproductive System of Male Rats

To explore the combined adverse effects of nonylphenol and soy isoflavone on the male offspring reproductive in rat.Methods] 50 pregnant rats were randomly divided into five groups (A:control group,B:nonylphenol group (200 mg/kg),C:nonylphenol low soy isoflavone dose (50 mg/kg)group,D:nonylphenol middle soy i soflavone dose (150 mg/kg)group,E:nonylphenol high soy isoflavone dose (450 mg/kg) group).Different dose of soy isoflavone and nonylphenol were administered to pregnant rats in each group by garage(as a solvent,only peanut oil was delivered to control group)during the period from 13th to 19th day of pregnant.FI offspring(male)were selected and sacrificed on 90~(th) day and their reproductive system was examined.Results]Compared with the control group,the activities of sperm,the sperm count in the tail of epididymis, the gonepoiesis quantity per day of the left testis,the weight and coefficient of testis,as well as ALP,LDH and TT in the group of soy isoflavone nonylphenol administered rats decreased significantly.Compared with nonylphenol treated group,the weight and coefficient of testis,the gonepoiesis quantity per day,ALP and LDH indices of F1 male rats in the nonylphenol 50 mg/kg soy isoflavone treated group increased significantly;the weight and coefficient of testis,the gonepoiesis quantity per day,ALP and LDH increased significantly,but the activity of sperm,the quantity of living offspring and TT showed the trend of decrease in the nonylphenol 450 mg/kg soy isoflavone treated group.Conclusion] Nonylphenol might cause hazards on the F1 male in differentiation period of reproductive system.The nonylphenol and 50 mg/kg soy isoflavone could show antagonistic effects,but the nonylphenol and 450 mg/kg soy isoflavone show additive actions on F1 male reproductive system of rats.