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| 冬凌草甲素对急性白血病原代细胞的增殖抑制作用 | |
| 引用本文： | 张俊峰,刘加军,陈规划,刘培庆,林东军,肖若芝,李旭东,黄仁魏.冬凌草甲素对急性白血病原代细胞的增殖抑制作用[J].热带医学杂志,2006,6(3):256-259. |
| 作者姓名： | 张俊峰 刘加军 陈规划 刘培庆 林东军 肖若芝 李旭东 黄仁魏 |
| 作者单位： | 1. 中山大学附属第三医院肝移植中心,广州,510630 2. 中山大学附属第三医院血液肿瘤科,广州,510630 3. 中山大学药学院,广州,510080 |
| 基金项目： | 国家重点发展研究计划“973”资助项目(No.2003CB515507)。 |
| 摘 要： | 目的研究冬凌草甲素对急性白血病原代细胞的增殖抑制作用。方法以人急性早幼粒细胞白血病(APL)患者的骨髓原代细胞为研究对象,以不同浓度的冬凌草甲素作用于体外培养的白血病细胞,MTT法检测细胞生长抑制率,应用流式细胞仪、台盼蓝染色及Hoechst33258荧光染色法观察细胞凋亡。结果冬凌草甲素体外可明显地抑制白血病原代细胞的增殖,并能诱导细胞发生凋亡,而且呈现出明显的量-效与时-效关系。结论冬凌草甲素能有效地抑制APL细胞的生长及诱导细胞发生凋亡,是一种潜在的抗白血病药物。 |
| 关 键 词： | 冬凌草甲素 白血病 原代细胞 细胞凋亡 |
| 文章编号： | 1672-3619（2006）03-0256-04 |
| 收稿时间： | 2005-11-13 |
| 修稿时间： | 2006-01-04 |
In Vitro Inhibitory Effect of Oridonin on Primary Acute Leukeamic Cells |
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| ZHANG Jun-feng,LIU Jia-jun,CHEN Gui-hua,LIU Pei-qing,LIN Dong-jun,XIAO Ruo-zhi,LI Xu-dong,HUANG Ren-wei.In Vitro Inhibitory Effect of Oridonin on Primary Acute Leukeamic Cells[J].Journal Of Tropical Medicine,2006,6(3):256-259. | |
| Authors: | ZHANG Jun-feng LIU Jia-jun CHEN Gui-hua LIU Pei-qing LIN Dong-jun XIAO Ruo-zhi LI Xu-dong HUANG Ren-wei |
| Abstract: | Objective To study the inhibitory effect of oridonin on primary acute leukeamic cells in vitro. Methods Primary acute promylocytic leukemia (APL) cells aspirated from the bone marrow of patients were used in this study. Isolated APL cells were incubated with various concentrations of Oridonin. The growth of the cells was measured by MTT method. Flow cytometry(FCM), typan-blue viability test,as well as Hoechst 33258 fluorescence dye were used to analyze the apoptotic cell death. Results Oridonin was found to inhibit the growth of APL and induce apoptosis in a time-and dose-dependent manner. Conclusion Oridonin can effectively inhibit the growth and induce apoptosis in primary APL cells. The possibility of using oridonin for the treatment of acute leukeamia should further be studied. |
| Keywords: | Oridonin leukeamia primary cell apoptosis |
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