散发性大肠管状腺瘤癌变过程中JNK1 Raf-1和Livin的表达及其意义

目的 探讨JNK1、Raf-1和Livin在散发性大肠管状腺瘤癌变过程中的可能作用.方法 采用免疫组化方法,检测21例正常大肠黏膜、65例散发性大肠管状腺瘤伴上皮不同程度异型增生及22例管状腺瘤癌变组织中,JNK1 、Raf-1和Livin的蛋白表达水平.结果 正常大肠黏膜组、散发性大肠管状腺瘤伴上皮异型增生组、大肠管状腺瘤癌变组,JNK1蛋白的阳性表达率分别为33.3%、63.1%和90.9%,Raf-1蛋白的阳性表达率分别为23.8%、52.3%和77.3%,Livin蛋白的阳性表达率分别为14.3%、52.3%和77.3%,大肠管状腺瘤伴上皮异型增生组和癌变组JNK1 Raf-1和Livin蛋白的阳性表达率均高于正常大肠黏膜组(均P＜0.05),而癌变组均高于大肠管状腺瘤伴上皮异型增生组(均P＜0.05).在大肠管状腺瘤伴上皮异型增生组中,Raf-1的阳性表达率随异型增生程度的增高而升高(P＜0.05),而上皮不同程度异型增生组间JNK1和Livin蛋白的阳性表达率差异无统计学意义(均P＞0.05).87例散发性大肠管状腺瘤组织中,JNK1、Raf-1和Livin蛋白表达两两正相关(JNK1和Raf-1:r=0.451,P＜0.05;JNK1和Livin:r于0.499,P＜0.05;Raf和Livin;r=0.445,P＜0.05).结论 JNK1、Raf-1和Livin在大肠管状腺瘤癌变过程中可能起一定作用.

Expression and significance of JNK1, Raf-1 and Livin in the carcinogenesis of sporadic colorectal tubular adenoma

Objective To study the possible role of JNK1, Faf-1 and Livin in the carcinogenesis of sporadic colorectal tubular adenoma Methods Immunohistochemical staining was used to detect the expression of JNK1, Raf-1 and Livin proteins in 65 sporadic colorectal tubular adenomaas with dysplasia of varying degrees and 22 colorectal tubular adenoma with cancerous area. Results In normal colorectal mucosa, colorectal tubular adenoma with dysplasia and colorectal tubular adenoma with cancerous area, the positive rate of JNK1, Raf-1 and Livin expression was in creased gradually.The positive expression of JNK1Raf-1 and Livin was all sighificantly higher in the cases of colorectal tubular adenoma with dysplasia or with cancerous area than that in normal colorectal mucosa ( P＜ 0.05), and the positive expression of JNK1, Raf-1and Livin was significantly higher in colorectal tubular adenoina with cancerous area than that in colorectal tubular adenoma with dvsplasia of different degrees (P ＜ 0 05). In the cases of colorectal tubulgr adenomawith dysplasia of varying degrees, the positive expression of Raf-1 was mcreased along with the increaslng dysplasia degrse of colorectal tubular adenoma (P＜0.05). Coexpressior of JNK1, Raf-1 and Livin increased gradually in the careinogenesis of sporadic colorectal tubular adenoma,while positive correlation was found among the expressions of JNK1, Raf-1 and Livin Conclusion JNK1,Raf-1 and Livin may be involved in the carciongenesis of sporadic colorectal tubular adenoma.