人白细胞相关抗原B27及细菌多肽在脊柱关节病发病中的作用

目的了解细菌多肽应具备什么基本条件才能被人白细胞相关抗原（ＨＬＡ）Ｂ２７递呈而导致脊柱关节病。方法以来自衣原体热休克蛋白６０、能与Ｂ２７分子结合的９肽作为原型，用人工方法将９肽的每一个氨基酸残基均以另外１９种氨基酸替换，将合成的１８０种９肽分别与Ｂ２７Ｔ２细胞孵育，观察其与抗Ｂ２７单克隆抗体Ｂ２７Ｍ２的反应性。结果与Ｂ２７分子结合的９肽其第２位氨基酸残基应是精氨酸，以其它１９种氨基酸替换均使Ｂ２７Ｍ２的反应性明显降低；第６，８，９位氨基酸残基的选择性亦较高，仅有６～８种氨基酸能诱导与Ｂ２７Ｍ２的反应性；第３，５，７位氨基酸残基没有什么选择性，几乎任何一种氨基酸残基对Ｂ２７Ｍ２反应性的影响均不大。结论第２，９位氨基酸残基是９肽与Ｂ２７紧密结合所必需的，而第６，８位氨基酸可能与抗Ｂ２７单克隆抗体的识别有密切关系

The inhibitory effect of antisense phosphorothioate c myc oligodeoxynucleotide on intimal hyperplasia in rat abdominal aortae after injury with a balloon catheter

Objective To evaluate the effect of Antisense phosphorothioate c myc oligodeoxynucleotide (ODN) on intimal hyperplasia in rat abdominal aortae induced by endothenial denuded injury with a balloon catheter. Methods Antisense c myc ODN was applied to the adventitia of injured abdominal aortae in rat introduced by pluronic gel releasing system and lipofectin delivery system. The animals were feeded with routine diet. Three weeks after intervention, the animals were killed and defined segments of arteries were sectioned and the histological sections were stained with elastic fiber staining. The cross section of the artery was examined microscopically and the image was digitalized by image analysis system. The intima/media(I/M) area and I/M thickness ratios were calculated and analyzed statistically among groups. Results The intimal hyperplasia in antisense c myc ODN treated rats was inhibited significantly, demonstrated by microscopically and I/M area and thichness ratios, as compared with that treated with sense c myc ODN and mismatch c myc ODN. The larger dose of antisense c myc ODN, the more obvious the suppression of intimal hyperplasia.Conclusion The results suggest antisense c myc ODN significantly suppressed the intimal hyperplasia in injured rat aortae in a sequence specific fashion and dose dependent manner. The investigation provides the basis for the early clinical trials assessing the therapeutic potential of antisense c myc approach for the prevention of restenosis after angioplasty.