河南食管癌高发区林州市同一个体食管贲门双源癌p53蛋白表达变化

目的 :探讨同一个体食管贲门双源癌组织p5 3蛋白变化的特征及其意义。方法 :采用免疫组化卵白素 生物素 过氧化物酶复合物 (ABC)法和组织病理学方法 ,分析河南食管癌高发区林州市 14例双源癌患者 (同时发生食管鳞癌和贲门腺癌 )p5 3蛋白的表达状况。结果 :在 14例双源癌患者中 ,食管鳞癌和贲门腺癌p5 3阳性率分别为 72 % (10 / 14 )和 3 6% (5 / 14 ) ,统计学分析二者间阳性率无显著性差异 (χ2 =2 2 85 ,P >0 0 5 )。同一个体食管和贲门癌组织p5 3一致性变化发生率占 5 0 % (一致阳性率为 2 9% ,一致阴性率为 2 1% )。统计学分析二者呈显著相关性 (P <0 0 5 )。结论 :本研究证实食管和贲门双源癌存在较高的p5 3一致性变化 ,提示二者可能具有相似的发病因素和分子机制

Alterations of p53 in concurrent carcinomas of the esophagus and gastric cardiafrom the same patient in Linzhou,Henan Province,a high incidence area for esophageal cancer

Objective: To characterize the alteration and significance of p53 in concurrent carcinomas of the esophagus and gastric cardia from the same patient.Methods: p53 protein accumulation in 14 patients with concurrent carcinoma of the esophagus and gastric cardia (occurring together with esophageal squamous cell carcinoma and gastric cardia adenocarcinoma) was detected by immunohistochemical method (ABC).Results: Of the 14 cases with concurrent cancers,p53 positive immunostaining rates in esophageal squamous cell carcinoma (SCC) and gastric cardia adenocarcinoma (GCA) were 72% (10/14) and 36% (5/14),respectively.The difference,however,was not significant ( χ 2=2.285,P >0.05).The coincidence rates for p53 positive staining in SCC and GCA from the same patients accounted for 50% (positive rate:29%;negative rate:21%).Correlation analysis showed significant relationship between the subgroups of coincidence negative or positive cases ( P <0.05).Conclusion: The present results demonstrated that there was a high frequency of p53 coincident changes in concurrent cancers of SCC and GCA,suggesting that there may be similar risk factors and molecular mechanisms involved in these SCC and GCA.