参青方对TNBS诱导的大鼠结肠炎模型结肠Cajal间质细胞的影响

背景：近年以Cajal间质细胞（ICC）为核心的肠道动力学与溃疡性结肠炎（UC）的关系逐渐成为UC研究的热点之一。目的：观察中药制剂参青方对三硝基苯磺酸（TNBS）诱导的大鼠结肠炎模型结肠组织中ICC形态和数量的影响，探讨该制剂调节UC肠道动力障碍的作用机制。方法：60只大鼠随机分为正常对照组、模型Ⅰ组、模型Ⅱ组、美沙拉秦组、参青方高剂量组和低剂量组，后5组以TNBS诱导结肠炎模型。模型Ⅰ组于造模后3d取材，其余5组于造模后3d开始予相应药物，连续给药7d后取材。电子显微镜观察病变结肠组织ICC超微结构，免疫组化染色和蛋白质印迹法检测c-kit蛋白表达。结果：模型Ⅰ组结肠组织ICC超微结构破坏明显，c-kit蛋白表达较正常对照组显著降低（P〈0．05）。经参青方或美沙拉秦治疗后，ICC超微结构异常有所改善，c-kit蛋白表达显著增高（P〈0．05），参青方高剂量组作用更为明显。结论：TNBS诱导的结肠炎大鼠结肠组织ICC超微结构损伤，数量减少，而参青方能减轻和修复ICC损伤并恢复其数量，对调节肠道动力起重要作用。

Influence of Shen-Qing Recipe on Colonic Interstitial Cells of Cajal in Rat Model with TNBS-induced Colitis

Background： Recently, the relevance of intestinal motility represented by interstitial cells of Cajal （ICC） and ulcerative colitis （UC） has become one of the loci in the studies of UC. Aims： To observe the influence of Shen-Qing Recipe （SQR）, a Chinese medicine on the morphology and amount of colonic ICC in rat model with trinitrobenzenesulfonie acid （TNBS）-induced colitis, and to investigate the mechanism of SQR in regulating the intestinal dysmotility in UC. Methods： Sixty rats were randomly divided into normal control group, model group Ⅰ, model group Ⅱ, mesalazine group, and high- dose and low-dose SQR groups. TNBS-indueed colitis model was constructed in the latter five groups. Colonic samples in model group Ⅰ were collected on the 3rd day after model construction, while in the other five groups, the samples were collected after treatment with corresponding agents for 7 consecutive days. Uhrastructure of ICC in the diseased colonic tissues was observed with electron microscope. Expression of c-kit protein was determined by immunohistochemical staining and Western blotting. Results： Ultrastructure of colonic ICC was injured markedly in rats of model group Ⅰ , and expression of c-kit protein decreased significantly as compared with that of normal controls （P〈0.05）. Treatment with SQR or mesalazine could improve the uhrastructure of ICC and increase the expression of c-kit protein （P〈0.05）, especially in the high-dose SQR group. Conclusions： The ultrastrueture of colonic ICC is injured and its amount was reduced in TNBS-induced rat colitis. SQR can alleviate and repair the injured ICC with recovery of the amount. It has an important role in modulating the intestinal motility.