体外培养诱导外周血淋巴细胞促进大鼠面神经再生的实验研究

目的:通过在神经缺损处局部回输体外分离培养纯化的淋巴细胞,了解此种方法促进面神经损伤修复的效果。方法:将20只Wistar大鼠的面神经颊支剪断并立即缝合(其余3支反折缝合)制成面神经损伤模型大鼠,将其分成淋巴细胞组和对照组,每组10只,每组再分成2周组和8周组。淋巴细胞组局部回输体外分离培养的外周血淋巴细胞,对照组作对照。于2周和8周测定面神经颊支-触须肌复合动作电位传导速度,辣根过氧化物酶(HRP)神经逆行示踪测定面神经核团的神经元阳性数目。结果:淋巴细胞组面神经颊支-触须肌复合动作电位传导速度8周时为0.64±0.07,与对照组(0.56±0.07)相比,差异有统计学意义(P<0.05)。HRP神经逆行示踪测定面神经核团神经元阳性数目,淋巴细胞组2周及8周与对照组同时间段相比差异均无统计学意义(均P>0.05)。结论:体外分离培养纯化的淋巴细胞在局部应用于神经损伤处对面神经再生修复可起一定的促进作用。

The effect of peripheral blood lyphmocyte cell cultured and induced in vitro on the facial nerve regeneration

OBJECTIVE: To explore the effect of peripheral blood lymphocyte cells cultured in vitro on the facial nerve repair and regeneration. METHOD: Facial nerves branch-ramus buccolabialis of 20 Wistar male rats were dissected and sutured immediately. Then they were divided into two groups randomly as lymphocytes transfusion group and control group (no cell transfusion). Each group was divided into two sub-groups as 2 w and 8 w. The action potential conduct velocity of the facial nerve was measured and the facial neuron number with HRP retrograde tracing method was counted. RESULT: In 8 weeks the action potential conduct velocity of the lymphocytes transfusion group was (0.64 +/- 0.07) m/s, which was significantly faster than that of the control group (0.56 +/- 0.07) m/s (P < 0.05). There was no difference of the facial nruron number among two sub-groups and the control group. CONCLUSION: When purified and amplified in vitro and auto-transfused around the injuried nerve, the peripheral blood lymphocyte cells may have some positive effects on facial nerve repair and regeneration.