| 脂多糖和IL-2对乙型肝炎疫苗体外诱导B细胞应答研究 |
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| 引用本文： | 邓文强,王萌,曾烈华,.脂多糖和IL-2对乙型肝炎疫苗体外诱导B细胞应答研究[J].中国医学工程,2008,16(2):81-83. |
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| 作者姓名： | 邓文强 王萌 曾烈华 |
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| 作者单位： | 1. 广东省韶关学院医学院,免疫病原学教研室,广东,韶光,512026
2. 广东省韶关市第一人民医院检验科,广东,韶光,512026 |
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| 摘 要： | 目的在乙型肝炎疫苗体外诱导B淋巴细胞产生HBs-Ab过程中，通过脂多糖和IL-2作为有效刺激物质，使用多秆组合方法进行观察比较，了解影响抗体产生的体外诱导因素。方法用乙型肝炎疫苗刺激组（A组）、乙型肝炎疫苗＋脂多糖刺激组（B组）、乙型肝炎疫苗＋脂多糖＋IL-2刺激组（C组）三组进行淋巴细胞培养和诱导，比较HBs-Ab产生情况，用酶联免疫吸附试验（ELISA）测定HBs-Ab含量。结果在脂多糖和IL-2的刺激诱导下。能有效促进B淋巴细胞产生HBs-Ab。结论实验证明脂多糖是激活人血B淋巴细胞的有效丝裂原，IL-2可促使活化B细胞增生及产生抗体，脂多糖和IL-2联合作用，可增强乙型肝炎疫苗诱导体液免疫水平。
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| 关 键 词： | 乙型肝炎疫苗 脂多糖(LPS) IL-2 B细胞 |
Effects of lipopolysaccharide and interleukin-2 on B lymphocyte response induced by hepatitis B vaccine in vitro |
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| Institution: | DENG Wen-qiang, WANG Meng, ZENG Lie-hua (1.Department of lmmunology and Microbiology, Medical College of Shaoguan University, Shaoguan, Guangdong 512026, P.R.China; 2.Department of Laboratory Medicine, the First People＇s Hospital of Shaoguan City, Shaoguan, Guangdong 512026, P.R.China) |
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| Abstract: | Objective] To investigate the effects of lipopolysaccharide (LPS) and interleukin-2 (IL-2) on HBs-Ab production in B lymphocyte induced by hepatitis B vaccine in vitro. Methods] B lymphocytes were incubated and stimulated by hepatitis B vaccine (A group), hepatitis B vaccine plus LPS (B group), or hepatitis B vaccine plus LPS and IL-2 (C group) respectively. HBs-Ab was determined by enzyme linked immunosorbent assay (ELISA) in three groups. Results] HBs-Ab was produced by B lymphocyte after the stimulati... |
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| Keywords: | hepatitis B vaccine lipopolysaccharide (LPS) interleukin-2 (IL-2) B lymphocyte |
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