矽尘致肺泡巨噬细胞氧化损伤的超微结构和细胞化学研究

目的：探讨矽尘致肺泡巨噬细胞损伤过程中是否存在着氧化损伤机制。方法：对矽尘作用于肺泡巨噬细胞产生的Ｏ＾－２和Ｈ２Ｏ２进行原位显示、定量测定并做相关分析；以ＡＴＰａｓｅ作为膜功能酶改变的反映；在亚细胞水平观察细胞形态结构。结果：细胞形态结构受损程度、膜ＡＴＰａｓｅ的活性与矽尘肺泡巨噬细胞早期产生的氧自由基活性直接相关。结论：（１）首次将原位显示氧自由基技术应用于矽尘致肺泡巨噬细胞损伤机制研究，发现氧

ULTRASTRUCTURAL AND CYTOCHEMICAL STUDY ON FREE RADICAL INJURY OF RAT ALVEOLAR MACROPHAGES BY SILICON DUST IN VITRO

To analyze the relationship between oxygen free radicals and the morphological damage of alveolar macrophages(AMs) induced by silicon dust. Mehtods: Cytochemical technqiue was used to demonstrate the oxygen radicals in situ. Meanwhile, the ultrastructure of AMs was observed. The cytochemical reaction of O2 and Mg2 -ATPase were analyzed quantitatively by image analyzer, and the linear correlation tendency between the quantitative results of the O2 and ATPase reactivity was elucidated. Rdults: Like opsonized zymosan A, toxic mineral particles, silicon dusts also could result in production of O2 and H2O2 on AMs. They were localized on the plasma membrane, the membrane of phagosome and/or pinosome and the lysosomal membrane. There was a close relationship between AMs morphological and enzymatic damage and the production of oxygen free radicals. Conclusion: (l) This paper firstly use the technique, which demonstrates the oxygen free radicals in situ to study the oxygen mechanism of the silicosis. Oxygen free radicals reaction induced by quartz and the free radical chain reaction is in one of the AMs damage mechanisms. Antioxidant may be given to patients to terminate oxygenic damage, and further protect evolvement of fibroblasts from proliferation. (2) Cytochemical technique to demonstrate O2 and H2O2 oxygen free radicals in situ is superior to other techniques in a sense. It is more convenient to observe the real situation of the oxidative damage on cells, making it easy to clarify the relationship between the production of free radicals and the damage degree on cells. It is a useful technique for studying the mechanisms of silicosis.