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| 紫花牡荆素体外抑制人卵巢癌HO-8910细胞增殖和诱导凋亡的研究 | |
| 引用本文： | 白军,谢宛玉,曹建国,夏红.紫花牡荆素体外抑制人卵巢癌HO-8910细胞增殖和诱导凋亡的研究[J].现代妇产科进展,2010,19(4). |
| 作者姓名： | 白军 谢宛玉 曹建国 夏红 |
| 作者单位： | 1. 南华大学第一附属医院,衡阳,421001 2. 湖南师范大学药物工程实验室 3. 南华大学肿瘤研究所 |
| 摘 要： | 目的:探讨紫花牡荆素(casticin)体外抑制人卵巢癌HO-8910细胞增殖和诱导凋亡的效应及其机制。方法:MTT法检测casticin对人卵巢癌HO-8910细胞增殖的抑制;Hoechest33258染色观察细胞凋亡形态学改变;流式细胞术(FCM)检测casticin处理HO-8910细胞的凋亡率;Western blotting分析caspase-3、CyclinB1、p21蛋白表达变化。结果:casticin对人卵巢癌HO-8910细胞增殖有较强的抑制作用,呈剂量和时间依赖性。经casticin作用48h后HO-8910细胞表现出典型的凋亡形态特征,并剂量依赖性地增加亚二倍峰,降低CyclinB1蛋白表达,增高caspase-3和p21表达。结论:casticin通过降低CyclinB1表达、活化p21和caspase-3抑制HO-8910细胞增殖并诱导凋亡。 |
| 关 键 词： | 卵巢肿瘤 细胞系 肿瘤 紫花牡荆素 凋亡 增殖 |
Effect of casticin on inhibition of proliferation and induction of apoptosis of human ovarian cancer HO-8910 cells in vitro |
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| Bai Jun,Xie Wanyu,Cao Jianguo,et al..Effect of casticin on inhibition of proliferation and induction of apoptosis of human ovarian cancer HO-8910 cells in vitro[J].Current Advances In Obstetrics and Gynecology,2010,19(4). | |
| Authors: | Bai Jun Xie Wanyu Cao Jianguo |
| Institution: | Bai Jun1,Xie Wanyu1,Cao Jianguo2,et al.1.The First Affiliated Hospital of the University of South China,Hengyang 421001,2.Laboratory of Medicine Engineering,Medical College,Hunan Normal University |
| Abstract: | Objective:To investigate the effect of casticin on inhibition of proliferation and apoptosis of human ovarian cancer HO-8910 cells in vitro.Methods:The inhibitory effect of casticin on the proliferation of human ovarian cancer HO-8910 cells was evaluated by the MTT assay and the apoptotic effect was demonstrated by Hoechst 33258 staining and flow cytometric analysis.The expressions of caspase-3,Cyclin B1,p21 were analyzed by Western blotting.Results:Casticin significantly inhibited the proliferation of huma... |
| Keywords: | Ovarian neoplasms Cell line tumor Casticin Apoptosis Proliferation |
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