p53 mutations in formaldehyde-induced nasal squamous cell carcinomas in rats. |
| |
Authors: | L Recio S Sisk L Pluta E Bermudez E A Gross Z Chen K Morgan C Walker |
| |
Institution: | Chemical Industry Institute of Toxicology, Research Triangle Park, North Carolina 27709. |
| |
Abstract: | Formaldehyde induces squamous cell carcinomas in the nasal passages of rats following chronic inhalation exposure at concentrations of > or = 10 ppm. We have examined the complementary DNA of the tumor suppressor gene p53 from 11 primary formaldehyde-induced tumors for mutation using DNA sequence analysis. A polymerase chain reaction-amplified fragment of the rat p53 complementary DNA containing the evolutionarily conserved regions II-V was directly sequenced from each tumor. Point mutations in the p53 complementary DNA sequence were found in 5 of 11 of the tumors analyzed. These data demonstrate p53 point mutations in formaldehyde-induced squamous cell carcinomas and indicate a common alteration in certain rat and human squamous cell carcinomas of the respiratory tract. |
| |
Keywords: | |
|