还原型谷胱甘肽对糖尿病大鼠肾组织非酶糖基化影响的试验研究

目的探讨还原型谷胱甘肽对糖尿病大鼠肾脏的保护作用及其可能机制。方法以链脲佐菌素诱导糖尿病大鼠模型，给予还原型谷胱甘肽、盐酸氨基胍单独或联合治疗8周，以荧光分光光度计测定肾皮质荧光值即糖基化终末产物（AGEs）含量，行肾组织PAS染色测定平均肾小球截面积（MGAl及体积（MGV），放免法测定尿白蛋白排泄率（UAER），同时检测糖化血红蛋白（HbAIc）、血清肌酐清除率（Cer）、肾重／体重比值、血糖水平等。结果8周时，各组糖尿病大鼠Ccr、UAER、MGA、MGV、肾重／体重比值、AGEs含量均较空白对照组明显增加（P〈0．05或P〈0．01）。还原型谷胱苷肽、盐酸氨基胍单独或联合治疗组Cor、UAER、MGA、MGV、肾重／体重比值、AGEs含量均较糖尿瘸对照组明显降低（P〈0．05或P〈0．01）。结论还原型谷胱苷肽及盐酸氨基胍均可减少肾组织糖基化终末产物生成，对糖尿病大鼠肾脏具保护作用。

Effect of reduced glutathione on nonenzymatic glycosylation in kidney of streptozotocin-induced diabetic rats

Objective To investigate the renoprotective effect of reduced glutathione on streptozotocin-induced diabetic rats as well as the possible mechanism involved in.Methods thods Streptozotocin-induced diabetic rats received aminoguanidine 50mgkg~(-1)d~(-1) and reduced glutathione 400mgkg~(-1)d~(-1) intraperitoneally respectively or synchronously for 8 weeks.The fluorescence of renal cortical tissue was measured by spectrofluorometer representing contents of AGEs.Urinary albumin excretion rate(UAER) was measured by radioimmunoassay.The mean glomerular area(MGA) and volume(MGV) vere measured by image analysis system.The changes of creatinine clearance rate(Ccr),the kidney weight/body weight ratio,the blood glucose and hemoglobinA1c levels were observed.Results By the end of 8 weeks,the Ccr,UAER,MGA,MGV,kidney weight/body weight ratio,and contents of AGEs increased significantly in DM groups compared with the blank control gloup(P<0.05 or P<0.01),and decreased significantly in aminoguanidine and reduced glutathione respectively or synchronously treatment groups compared with the DM control gloup(P<0.05 or P<0.01).Conclusion Reduced glutathione and aminoguanidine can inhibit the overproduction of AGEs in diabetic rats,and it's renoprotective mechanism varrants further investigation.