| 抗HER2单链抗体融合凋亡蛋白对SKOV3细胞的靶向杀伤 |
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| 引用本文： | 张立红,贾林涛,鲍炜,于翠娟,赵晶,金明,王成济,杨安钢.抗HER2单链抗体融合凋亡蛋白对SKOV3细胞的靶向杀伤[J].细胞与分子免疫学杂志,2003,19(2):160-162. |
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| 作者姓名： | 张立红 贾林涛 鲍炜 于翠娟 赵晶 金明 王成济 杨安钢 |
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| 作者单位： | 第四军医大学基础部生物化学与分子生物学教研室,陕西,西安,710032 |
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| 基金项目： | 国家高技术发展计划 (863)资助项目 (No.2 0 0 1AA2 1 71 0 1 )，国家杰出青年科学基金资助项目 (No.399Z50 36)，军队杰出中青年人才基金资助项目 (No .98J0 0 9) |
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| 摘 要： | 目的：探讨分泌表达的抗HER2单链抗体与重构型人caspase—3融合蛋白对HER2抗原阳性肿瘤细胞的靶向杀伤作用。方法：将重构型人caspase—3基因亚克隆人pCMV—eDscFv—PEII—PEIII的相应位点，构建重组真核表达载体pCMV—e23scFv—PEII—revcasP3，并转染人T淋巴瘤细胞系Jurkat，筛选并建系。用ELISA检测培养上清中融合蛋白的分泌表达。通过共培养实验观察含有该融合蛋白的培养上清对人卵巢癌细胞SKOV3生长的抑制作用。结果：融合蛋白基因可在Jurkat细胞中，分泌表达并杀伤SKOV3细胞。结论：分泌表达的抗erbB2单链抗体与重构型人caspase3融合蛋白能够靶向诱导SKOV3细胞死亡。
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| 关 键 词： | 天冬氨酸特异性半胱氨酸蛋白酶 单链抗体 表皮生长因子受体 HER－2 肿瘤 SKOV3细胞 靶向杀伤作用 |
| 文章编号： | 1007-8738(2003)02-160-03 |
Expression of a secreted fusion protein consisting of anti-HER2 antibody and reversed caspase-3 and its suppression on growth of SKOV3 cells |
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| Li-Hong Zhang,Lin-Tao Jia,Wei Bao,Cui-Juan Yu,Jing Zhao,Ming Jin,Cheng-Ji Wang,An-Gang Yang.Expression of a secreted fusion protein consisting of anti-HER2 antibody and reversed caspase-3 and its suppression on growth of SKOV3 cells[J].Journal of Cellular and Molecular Immunology,2003,19(2):160-162. |
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| Authors: | Li-Hong Zhang Lin-Tao Jia Wei Bao Cui-Juan Yu Jing Zhao Ming Jin Cheng-Ji Wang An-Gang Yang |
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| Institution: | Department of Biochemistry and Molecular Biology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710032, China. |
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| Abstract: | AIM: To investigate the killing effect on SKOV3 cells by fusion protein HER2 specific antibody reversed caspase 3 in the secreted form. METHODS: The reversed human caspase 3 gene was subcloned into pCMV e23scFv PEII PEIII to construct recombinant eukaryotic expression vector pCMV e23scFv PEII revcasp 3 and transfect Jurkat cells. The secreted expression of the in culture supernant of transfected Jurkat cells was detected by ELISA. The suppression of the fusion protein on growth of SKOV3 cells was examined by co culture with supernant of transfected Jurkat cells. RESULTS: There was the secreted expression of the fusion protein in Jurkat cells. Expressed product had the activity of inducing SKOV3 cells to death. CONCLUSION: The fusion protein expressed in the secreted form can targeted towards SKOV3 cells and kill them. |
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| Keywords: | reversed caspase 3 HER2 secreted expression targeted cytotoxicity |
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