孕妇血清和羊水中IL-8、MMP-9检测在诊断胎膜早破并绒毛膜羊膜炎中的价值

目的]探讨孕妇血清及羊水中白细胞介素8（IL-8）、基质金属蛋白酶-9（MMP-9）检测对判断绒毛膜羊膜炎的临床价值。【方法】采用酶联免疫吸附法检测30例胎膜早破及40例正常孕妇母血、羊水中IL-8、MMP9水平的变化，同时进行胎膜的病理检查。【结果】胎膜早破组母血、羊水中IL-8、MMP-9的含量均高于对照组（P〈0．01或P〈0．05）。母血、羊水中IL-8、MMP-9的含量随破膜时间的延长而增高，〉24h增高尤为明显。绒毛膜羊膜炎患者母血、羊水IL-8、MMP-9水平明显高于非绒毛膜羊膜炎患者（P〈0．05，P〈0．01）。【结论】孕妇血清和羊水中IL-8、MMP-9水平升高可作为临床诊断早期绒毛膜羊膜炎的指标。

Clinical Significance of the Levels of IL-8 and MMP-9 in Maternal Serum and Amniotic Fluid in Patients with Premature Rupture of Membranes Complicated with Chorioamnionitis

Objective] To investigate the clinical roles of matrix metalloproteinase-9 （MMP-9） and inter-leukin-8（IL-8） in predicting premature rupture of membranes （PROM） and chorioamnionitis . Methods]The levels of IL-8 and MMP-9 were detected by enzyme linked immunosorbent assay in maternal serum and amniotic fluid of 30 pregnant women with PROM and 40 women with normal pregnancies. Chorioamnionitis was his-topathologically confirmed after delivery. Results]The levels of IL-8 and MMP-9 were significantly higher in amniotic fluid in patients with PROM than those of the control group （P〈0.01） . The levels of MMP-9 was significantly higher in maternal serum in patients with PROM than those of the control group （P〈0.05） . The longer the duration from rupture of membrance to delivery it was, the more serious chorioamnionitis it had, and the higher the levels of IL-8 and MMP-9 in maternal serum and amniotie fluid were. The levels of IL- 8 and MMP-9 were significantly higher in maternal serum and amniotie fluid in patients with ehorioamnionitis than those of non-ehorioamnionitis（P〈0.05, P〈0.01）. Conclusion]PROM is associated with the in- creased IL-8 and MMP-9 levels . IL-8 and MMP-9 are the valuable clinical biological markers for identifying the chorioamnionitis.