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Molecular analysis of coat protein coding region of tobacco stunt virus shows that it is a strain of <Emphasis Type="Italic">Lettuce big-vein virus</Emphasis> in the genus <Emphasis Type="Italic">Varicosavirus</Emphasis>
Authors:T Sasaya  K Ishikawa  S Kuwata  H Koganezawa
Institution:(1) Laboratory of Plant Virology, National Agricultural Research Center for Western Region, Zentsuji, Japan;(2) Department of Life Science, Meiji University, Kawasaki, Japan;(3) Kunisada Plant Breeding Station, Kaneko Seeds Co. Ltd., Akabori, Gunma, Japan
Abstract:Summary. To evaluate the relationship between tobacco stunt virus (TStV) and Lettuce big-vein virus (LBVV), we determined nucleotide sequences of the coat protein (CP) coding region of five TStV and three LBVV isolates and compared them with those of one Japanese and four Spanish isolates of LBVV. CP coding regions were identical in size and the nucleotide and amino acid sequence identities between TStV and LBVV were 95.6–96.5% and 97.2–98.7%, respectively. Phylogenetic analysis of nucleotide sequences indicated that TStV was very closely related to LBVV and a strain of LBVV rather than a distinct species.
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