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| 胚胎嗅鞘细胞和中脑腹侧细胞移植对帕金森病SD大鼠纹状体神经元存活和分化的影响 | |
| 引用本文： | 任玉水,陈琳,黄红云,周长满,刘佳,肖娟,王洪美,郗海涛,田国忠.胚胎嗅鞘细胞和中脑腹侧细胞移植对帕金森病SD大鼠纹状体神经元存活和分化的影响[J].解剖科学进展,2010,16(5). |
| 作者姓名： | 任玉水 陈琳 黄红云 周长满 刘佳 肖娟 王洪美 郗海涛 田国忠 |
| 作者单位： | 1. 北京康复中心神经外科,北京,100144;北京市虹天济神经科学研究院,北京,100144 2. 北京康复中心神经外科,北京,100144;北京大学医学部解剖与组织胚胎学系,北京,100191 3. 北京康复中心神经外科,北京,100144 4. 北京市虹天济神经科学研究院,北京,100144 |
| 基金项目： | 北京市优秀人才培养资助基金 |
| 摘 要： | 目的观察胚胎嗅球嗅鞘细胞(OECs)和胚胎中脑腹侧细胞(VMCs)联合移植对帕金森病(PD)SD大鼠纹状体神经元存活及分化的影响。方法 12只PD模型SD大鼠随机平均分成两组:单独VMCs组(在脑立体定位仪下将VMCs植入PD大鼠模型毁损侧纹状体内)和联合移植组(在脑立体定位仪下将OECs和VMCs植入PD大鼠模型毁损侧纹状体内)。OECs来源于5～7d绿荧光鼠的嗅球嗅鞘,VMCs来源于孕13～14d绿荧光胎鼠中脑腹侧的脑组织。于移植后4、14周灌注取脑,切片后做酪氨酸羟化酶(TH)免疫组织化学染色,检测胚胎中脑腹侧细胞的存活及分化状况。结果移植4周后单独VMCs组及联合移植组纹状体区都可检测到TH免疫阳性神经元,数量无统计学差异(P0.05)。移植14周后联合移植组纹状体区TH免疫阳性神经元数量比单独VMCs组明显增多(P0.05)。结论胚胎中脑腹侧细胞联合嗅鞘细胞移植入帕金森病大鼠纹状体促使纹状体神经元分化为多巴胺能神经元。 |
| 关 键 词： | 嗅鞘细胞 中脑腹侧细胞 帕金森病 酪氨酸羟化酶 SD大鼠 |
Effect of coimplantation of embryonic olfactory ensheathing cells and ventral mesencephalic cells on the survival and differentiation of striatum neurons in Parkinson's disease rats |
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| REN Yu-shui,CHEN Lin,HUANG Hong-yun,ZHOU Chang-man,LIU Jia,XIAO Juan,WANG Hong-mei,XI Hai-tao,TIAN Guo-zhong.Effect of coimplantation of embryonic olfactory ensheathing cells and ventral mesencephalic cells on the survival and differentiation of striatum neurons in Parkinson's disease rats[J].Progress of Anatomical Sciences,2010,16(5). | |
| Authors: | REN Yu-shui CHEN Lin HUANG Hong-yun ZHOU Chang-man LIU Jia XIAO Juan WANG Hong-mei XI Hai-tao TIAN Guo-zhong |
| Institution: | REN Yu-shui~(1,2),CHEN Lin~(1,2*),HUANG Hong-yun~(1,ZHOU Chang-man~(1,3),LIU Jia~1,XIAO Juan~2,WANG Hong-mei~(1,XI Hai-tao~1,TIAN Guo-zhong~2 (1.Department of Neurosurgery,Beijing Rehabilitation Center,Beijing 100144,2.Beijing Hongtianji Neuroscience Academy,3.Department of Anatomy & Embryology,Peking University Health Science Center,Beijing 100191 China) |
| Abstract: | Objective To observe the effect of coimplantation of embryonic olfactory ensheathing cells(OECs) and ventral mesencephalic cells(VMCs) on the survival and differentiation of striatum neurons in Parkinson's disease(PD) of rats.Method 12 SD rats were divided equally into two groups at random:VMCs(from ventral mesencephalic tissue of pregnant 13-14d green fluorescent fetal rat) group and the OECs(derived from the olfactory bulb of 5-7d green fluorescent rat) + VMCs group.4 and 14 weeks after transplantation,br... |
| Keywords: | olfactory ensheathing cells ventral mesencephalic cells Parkinson's disease tyrosine hydroxylase SD rat |
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