Isolation of a Chlamydomonas reinhardtii telomer by functional complementation in yeast |
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Authors: | T Hails O Huttner A Day |
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Institution: | (1) Genetics Laboratory, Biochemistry Department, Oxford University, South Parks Road, OX1 3QU Oxford, UK;(2) School of Biological Sciences, Manchester University, 3.614 Stopford Building, Oxford Road, M13 9PT Manchester, UK |
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Abstract: | We attempted to determine whether Chlamydomonas reinhardtii telomeres, which do not form G-quartet structures readily in vitro, are able to nucleate telomere addition in Saccharomyces cerevisiae. Restricted C. reinhardtii genomic DNA was ligated to a linear S. cerevisiae vector lacking a telomere. A C. reinhardtii telomere ligated to this unprotected end allowed vector replication as a linear DNA molecule in S. cerevisiae. DNA sequencing revealed common T4AG3]n and variant T6AG3 and T5AG3
C. reinhardtii telomere repeats capped by S. cerevisiae telomere repeat units. The recognition of a C. reinhardtii telomere by the telomere maintenance machinery of S. cerevisiae is consistent with a common theme for telomere structure in organisms with divergent telomere repeats. |
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Keywords: | Telomere Functional complementation C reinhardtii S cerevisiae |
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