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何首乌炮制前、后水溶性糖含量的比较研究
引用本文:丘小惠,李建华,黄志海. 何首乌炮制前、后水溶性糖含量的比较研究[J]. 中国药房, 2006, 17(12): 954-956
作者姓名:丘小惠  李建华  黄志海
作者单位:1. 广州中医药大学第二临床医学院,广州市,510120
2. 广东康美药业股份有限公司,普宁市,515300
摘    要:目的比较何首乌炮制前、后水溶性糖的含量。方法采用硫酸-蒽酮比色法测定何首乌生品及其炮制品中水溶性总糖、单糖-低聚糖、多糖的含量。结果葡萄糖检测浓度在0.06~0.60mg/ml范围内与吸收度线性关系良好;水溶性总糖、单糖-低聚糖平均回收率分别为99.2%(RSD=2.3%)、99.7%(RSD=2.5%)。结论何首乌炮制后水溶性总糖含量升高,其中单糖-低聚糖、多糖均有所增加,以多糖含量增加为主。

关 键 词:何首乌  炮制  硫酸-蒽酮比色法  水溶性糖
文章编号:1001-0408(2006)12-0954-03
收稿时间:2005-10-20
修稿时间:2006-01-04

Content Comparative Analysis of Water Soluble Saccharides in Radix Polygoni Multiflori before and after Its Processing
QIU Xiaohui,LI Jianhua,HUANG Zhihai. Content Comparative Analysis of Water Soluble Saccharides in Radix Polygoni Multiflori before and after Its Processing[J]. China Pharmacy, 2006, 17(12): 954-956
Authors:QIU Xiaohui  LI Jianhua  HUANG Zhihai
Affiliation:1.The Second Clinical College of Guangzhou University of TCM, Guangzhou 510120, China;2.Guangdong Kangmei Pharmaceutical Co.Ltd. ,Puning 515300,China
Abstract:OBJECTIVE:To compare the content of water-soluble saccharides in Radix Polygoni Multiflori before and after its processing.METHODS:The content of all water soluble saccharides,monose-oligosaccharides,polysaccharides in Radix Polygoni Multiflori and its processed products were determined by sulphuric acid-anthrone colorimetry.RESULTS:The detectable concentration of glucose showed a good linearity with absorbability in the range of 0.06~0.60mg/ml.The average recovery rates of water-soluble saccharides and monose-oligosaccharides were 99.2%(RSD=2.3%) and 99.7%(RSD=2.5%),respectively.CONCLUSION:The content of saccharides in processed Radix Polygoni Multiflori increased,including monose-oligosaccharides and polysaccharides,especially polysaccharides.
Keywords:Radix Polygoni Multiflori  Processing  Sulphuric acid- anthrone colorimetry  Water soluble saccharides
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