生长期缺锌对大鼠血清元素的影响

目的:研究膳食锌缺乏对生长期大鼠血清各种元素的影响。方法:刚断奶的SD大鼠,按体重相近配伍,随机分为3组,每组8只:缺锌组(ZD),配饲组(PF),对照组(ZA)。缺锌饲料、正常饲料含锌量分别为0.4mg/kg3、0 mg/kg,实验期15 d。用等离子电感耦合质谱仪(ICP—MS)测定大鼠血清元素锌、铁、铜、硒、锰、钴、铬、钙、镁、镉的含量。结果:缺锌组大鼠:血清铁(573.86±201.71μg/L)明显低于配饲组(1372.37±118.69μg/L)和正常组(1463.55±331.66μg/L)(P<0.05);血清锰(12.28±5.33μg/L)明显高于配饲组(4.24±0.73μg/L)和正常组(3.10±1.10μg/L)(P<0.05);血清铜(534.71±113.77μg/L)、硒(262.55±38.57μg/L)、镁(25.26±9.29μg/dl)低于正常组(铜725.91±158.27μg/L,硒465.90±132.18μg/L,镁39.84±14.10μg/dl)(P<0.05),与配饲组无明显差异(铜566.86±134.96μg/L,硒336.71±112.30μg/L,镁29.59±6.99μg/dl)(P>0.05);相比于配饲组与正常组,血清钙有下降趋势,但差异无显著性(P>0.05),血清钴、铬、镉有升高趋势,但差异无显著性(P>0.05)。结论:膳食锌缺乏影响生长期大鼠体内的微量元素水平。

Effect of zinc deficiency on serum elements in growing rats

Objectives： To investigate the effect of zinc deficiency on elements in growing rats. Methods： 24 weaning SD rats were randomly divided into three groups according to weight ： zinc deficiency （ ZD）, paired - fed （ PF）, zinc adaquation （ZA）. After 15 days feeding,took the serum to test the concentration of elements including zinc, iron, copper, selenium, manganum, cobalt, chrome, calcium, magnesium, cadmium with ICP--MS. Results：The iron concentration of ZD group were significantly lower than those of PF group and ZA group （573.86±201.71）±g/L ＆ （1372.37± 118.69） μg /L ＆ （1463.55±331.66） μg /L,P〈0.05].The manganum concentration of ZD group were significantly higher than those of PF group and ZA group （12.28 ±5.33） μg/L ＆ （4.24 ±0.73）μg/L ＆ （3.10± 1.10） μ g/L,P〈0.05].The concentration of copper（534.71 ± 113.77 μg/L）, selenium（262.55 ± 38.57μg/L） and magnesium（25.26 ± 9.29 μg/dl） in ZD group were significantly lower than those of ZA group （725.91 ± 158.27） μg/L ＆ （465.90± 132.18） μg /L＆ （39.84±14.10） μg/dl, P 〈 0. 05], while The concentrations of these three elements had no significantly difference between the ZD group and PF group（ P 〉 0.05）. Comparing with the PF and ZA groups, the concentration calcium in the ZD group is lower and the concentrations of cobalt, chrome and cadmium were higher, but the difference was not significant（ P 〉 0.05）. Conclusions ： zinc deficiecy alters the serum elements concentrations.