| 中药苦参碱对K562细胞骨架结构与功能的影响(英文) |
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| 引用本文： | 张彦,刘小珊,何於娟,马凌娣,许相儒,蒋纪恺. 中药苦参碱对K562细胞骨架结构与功能的影响(英文)[J]. 中国组织工程研究与临床康复, 2003, 7(20): 2788-2789 |
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| 作者姓名： | 张彦 刘小珊 何於娟 马凌娣 许相儒 蒋纪恺 |
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| 作者单位： | 重庆医科大学检验系临床生化教研室,重庆市,400016 |
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| 基金项目： | 国家自然科学基金(30171150)~~ |
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| 摘 要： | 目的从细胞骨架方面进一步探索苦参碱对人白血病K562细胞株的作用机制。方法0.1g/L苦参碱处理K562细胞24h前后,采用微管吮吸技术检测其黏弹系数的改变,并运用基因芯片技术分析细胞骨架蛋白基因表达的改变情况。结果0.1g/L苦参碱作用K562细胞24h后:弹性系数K1由725.7±215.1下降432.5±67.2;弹性系数K2由433.1±119.3下降242.5±31.2;黏性系数μ由71.5±38.0下降至49.5±15.3。细胞骨架类基因prefoldin与ezrin的mRNA表达增强。结论苦参碱能够导致K562细胞的骨架结构与功能发生变化。
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| 关 键 词： | 苦参碱 基因 K562细胞 |
Effects of matrine on structure and function of K562 cells |
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| Yan Zhang,Xiao-Shan Liu,Yu-Juan He,Ling-Di Ma,Xiang-Ru Xu,Ji-Kai Jiang. Effects of matrine on structure and function of K562 cells[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2003, 7(20): 2788-2789 |
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| Authors: | Yan Zhang Xiao-Shan Liu Yu-Juan He Ling-Di Ma Xiang-Ru Xu Ji-Kai Jiang |
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| Affiliation: | Yan Zhang,Xiao-Shan Liu,Yu-Juan He,Ling-Di Ma,Xian g-Ru Xu,Ji-Kai Jiang,Department of Clinical Biochemistr y,Chongqing Medica l University,Chongqing 400046,China |
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| Abstract: | Aim To investigate the effect of matrine on cytoskeletonof K562 cells. Methods Micropipette aspiration technique was adoptedto investigate the viscoelasticity of K562 cells, while the different ex-pression of cytoskeletal protein gene was analyzed by DNA microar-ray. Results In matrine-treated K562 cells, the viscoelastic propertiesKI, K2 and were decreased significantly from 726 ± 215 to 432 ±67,433 ±119 to 242±31, 72±38 to 50±15 respectively, and the geneexpression of prefoldin and ezrin was much stronger than that of controlcells. Conclusion The strueture and function can be changed in ma-trine-treated K562 cells. |
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| Keywords: | natrine genes k562 cells |
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